This gene encodes a protein that belongs to the pyrroline-5-carboxylate reductase family of enzymes. Members of this family catalyze the final step in proline biosynthesis, converting pyrroline-5-carboxylate to proline. Glutamate and ornithine are precursors in the synthesis of proline. The protein encoded by this gene is a cytoplasmic enzyme involved in the biosynthesis of proline from ornithine. [provided by RefSeq, Aug 2016]
Transcription factors with Perturb-seq knockdown data for PYCR3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PYCR3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PYCR3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:143,334,216–143,335,267 | 274.7 kb | Distal (>10kb) Multiome | 688 | |
| chr8:143,368,147–143,369,893 | 240.9 kb | Distal (>10kb) Multiome | 698 | |
| chr8:143,407,212–143,408,612 | 201.5 kb | Distal (>10kb) Multiome | 447 | |
| chr8:143,428,968–143,431,754 | 178.7 kb | Distal (>10kb) Multiome | 743 | |
| chr8:143,540,714–143,542,061 | 68.1 kb | Distal (>10kb) Multiome | 849 | |
| chr8:143,553,122–143,554,177 | 55.9 kb | Distal (>10kb) Multiome | 775 | |
| chr8:143,558,225–143,558,936 | 51.1 kb | Distal (>10kb) Multiome | 600 | |
| chr8:143,567,166–143,570,227 | 40.4 kb | Distal (>10kb) Multiome | 485 | |
| chr8:143,571,367–143,572,283 | 37.8 kb | Distal (>10kb) Multiome | 443 | |
| chr8:143,577,641–143,578,799 | 31.3 kb | Distal (>10kb) Multiome | 470 | |
| chr8:143,596,744–143,599,643 | 12.0 kb | Distal (>10kb) Multiome | 973 | |
| chr8:143,608,979–143,610,357 | 134 bp | At TSS Multiome | 735 | |
| chr8:143,617,068–143,618,380 | 8.0 kb | Proximal (<10kb) Multiome | 823 | |
| chr8:143,635,715–143,636,601 | 26.4 kb | Distal (>10kb) Multiome | 786 | |
| chr8:143,716,075–143,716,913 | 106.7 kb | Distal (>10kb) Multiome | 311 | |
| chr8:143,733,345–143,734,440 | 124.3 kb | Distal (>10kb) Multiome | 663 | |
| chr8:143,740,039–143,740,754 | 130.9 kb | Distal (>10kb) Multiome | 308 | |
| chr8:143,771,202–143,772,221 | 162.3 kb | Distal (>10kb) Multiome | 405 | |
| chr8:143,814,945–143,816,493 | 206.1 kb | Distal (>10kb) Multiome | 893 | |
| chr8:143,828,724–143,829,946 | 219.8 kb | Distal (>10kb) Multiome | 933 | |
| chr8:143,840,284–143,841,979 | 231.5 kb | Distal (>10kb) Multiome | 551 | |
| chr8:143,877,814–143,879,346 | 268.9 kb | Distal (>10kb) Multiome | 444 |
Genomic view of the PYCR3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.