PURB

purine rich element binding protein B | PURBETA

This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]

Member of: DE-5

Biological processes 32 terms

DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor binding (GO:0140297)DNA-binding transcription factor binding (GO:0140297)DNA-binding transcription repressor activity, RNA polymerase II-specific (GO:0001227)DNA-binding transcription repressor activity, RNA polymerase II-specific (GO:0001227)RNA binding (GO:0003723)RNA binding (GO:0003723)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)SMAD binding (GO:0046332)double-stranded DNA binding (GO:0003690)mRNA binding (GO:0003729)mRNA binding (GO:0003729)mRNA regulatory element binding translation repressor activity (GO:0000900)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of translation (GO:0017148)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)purine-rich negative regulatory element binding (GO:0032422)purine-rich negative regulatory element binding (GO:0032422)purine-rich negative regulatory element binding (GO:0032422)regulation of myeloid cell differentiation (GO:0045637)regulation of transcription by RNA polymerase II (GO:0006357)single-stranded DNA binding (GO:0003697)single-stranded DNA binding (GO:0003697)

Expression (TPM)

TFs regulating PURB 0 TFs

Transcription factors with Perturb-seq knockdown data for PURB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PURB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to PURB

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PURB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr7:44,581,475–44,583,147	303.2 kb	Distal (>10kb) Multiome HiCAR	864
chr7:44,606,125–44,607,487	278.7 kb	Distal (>10kb) Multiome	1043
chr7:44,748,085–44,749,703	136.9 kb	Distal (>10kb) Multiome	665
chr7:44,795,796–44,797,747	89.0 kb	Distal (>10kb) Multiome	1094
chr7:44,847,376–44,848,800	37.4 kb	Distal (>10kb) Multiome	990
chr7:44,884,668–44,886,169	43 bp	At TSS Multiome	956
chr7:44,962,460–44,963,298	77.4 kb	Distal (>10kb) Multiome	179
chr7:44,986,037–44,987,077	101.2 kb	Distal (>10kb) Multiome	748
chr7:44,999,127–45,000,858	114.4 kb	Distal (>10kb) Multiome	706
chr7:45,051,984–45,052,624	166.8 kb	Distal (>10kb) Multiome	90
chr7:45,088,228–45,089,744	203.7 kb	Distal (>10kb) Multiome	375
chr7:45,111,371–45,112,159	226.3 kb	Distal (>10kb) Multiome	1026

Genome Browser

Genomic view of the PURB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.