PURA

purine rich element binding protein A | PUR-ALPHA, PUR1, PURALPHA

This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]

Developmental clusters: GC6

Biological processes 40 terms

DNA binding (GO:0003677)DNA replication initiation (GO:0006270)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor binding (GO:0140297)DNA-binding transcription factor binding (GO:0140297)DNA-binding transcription repressor activity, RNA polymerase II-specific (GO:0001227)DNA-binding transcription repressor activity, RNA polymerase II-specific (GO:0001227)RNA binding (GO:0003723)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)SMAD binding (GO:0046332)chromosome, telomeric region (GO:0000781)cytoplasm (GO:0005737)dendrite (GO:0030425)dendritic transport of messenger ribonucleoprotein complex (GO:0098963)double-stranded DNA binding (GO:0003690)double-stranded telomeric DNA binding (GO:0003691)glutamatergic synapse (GO:0098978)mRNA regulatory element binding translation repressor activity (GO:0000900)mRNA regulatory element binding translation repressor activity (GO:0000900)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of translation (GO:0017148)neuronal cell body (GO:0043025)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)postsynapse (GO:0098794)protein binding (GO:0005515)purine-rich negative regulatory element binding (GO:0032422)purine-rich negative regulatory element binding (GO:0032422)purine-rich negative regulatory element binding (GO:0032422)regulation of transcription by RNA polymerase II (GO:0006357)single-stranded DNA binding (GO:0003697)single-stranded DNA binding (GO:0003697)single-stranded DNA binding (GO:0003697)transcription regulator inhibitor activity (GO:0140416)

Expression (TPM)

TFs regulating PURA 0 TFs

Transcription factors with Perturb-seq knockdown data for PURA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PURA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to PURA

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PURA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr5:139,847,851–139,848,888	259.5 kb	Distal (>10kb) Multiome	252
chr5:139,897,165–139,897,870	210.3 kb	Distal (>10kb) Multiome	20
chr5:139,903,738–139,904,936	203.5 kb	Distal (>10kb) Multiome	298
chr5:139,955,312–139,956,123	152.0 kb	Distal (>10kb) Multiome	118
chr5:139,968,615–139,969,112	138.8 kb	Distal (>10kb) Multiome	565
chr5:140,038,152–140,038,948	69.2 kb	Distal (>10kb) Multiome	241
chr5:140,042,572–140,043,835	64.5 kb	Distal (>10kb) Multiome	523
chr5:140,107,138–140,108,849	92 bp	At TSS Multiome	993
chr5:140,111,252–140,111,490	3.5 kb	Proximal (<10kb)	69
chr5:140,112,921–140,114,941	6.3 kb	Proximal (<10kb) Multiome	811
chr5:140,145,842–140,146,580	38.3 kb	Distal (>10kb) Multiome	366
chr5:140,157,178–140,157,756	49.6 kb	Distal (>10kb) Multiome	504
chr5:140,174,682–140,176,221	67.3 kb	Distal (>10kb) Multiome	959
chr5:140,302,631–140,303,538	195.4 kb	Distal (>10kb) Multiome	552
chr5:140,345,869–140,347,147	238.9 kb	Distal (>10kb) Multiome	671
chr5:140,363,055–140,363,697	255.6 kb	Distal (>10kb) Multiome	542
chr5:140,372,779–140,373,538	265.4 kb	Distal (>10kb) Multiome	302
chr5:140,400,395–140,402,925	293.8 kb	Distal (>10kb) Multiome	1129

Genome Browser

Genomic view of the PURA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.