The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
Transcription factors with Perturb-seq knockdown data for PSMA5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PSMA5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PSMA5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:109,213,499–109,214,619 | 212.6 kb | Distal (>10kb) Multiome | 770 | |
| chr1:109,229,475–109,230,215 | 196.6 kb | Distal (>10kb) Multiome | 221 | |
| chr1:109,240,604–109,241,116 | 185.4 kb | Distal (>10kb) Multiome | 391 | |
| chr1:109,249,055–109,250,364 | 176.9 kb | Distal (>10kb) Multiome HiCAR | 527 | |
| chr1:109,263,250–109,264,042 | 162.7 kb | Distal (>10kb) Multiome | 767 | |
| chr1:109,282,693–109,283,858 | 143.2 kb | Distal (>10kb) Multiome | 850 | |
| chr1:109,306,995–109,308,024 | 118.8 kb | Distal (>10kb) Multiome | 425 | |
| chr1:109,397,369–109,398,671 | 28.4 kb | Distal (>10kb) Multiome | 654 | |
| chr1:109,425,842–109,427,054 | 111 bp | At TSS Multiome | 922 | |
| chr1:109,466,294–109,467,385 | 40.2 kb | Distal (>10kb) Multiome | 452 | |
| chr1:109,483,147–109,484,806 | 57.5 kb | Distal (>10kb) Multiome | 1031 | |
| chr1:109,493,695–109,494,597 | 67.7 kb | Distal (>10kb) Multiome | 842 | |
| chr1:109,498,180–109,498,608 | 72.0 kb | Distal (>10kb) Multiome | 178 | |
| chr1:109,509,196–109,510,011 | 83.2 kb | Distal (>10kb) Multiome | 448 | |
| chr1:109,532,157–109,533,159 | 106.3 kb | Distal (>10kb) Multiome | 517 | |
| chr1:109,548,385–109,549,096 | 122.2 kb | Distal (>10kb) Multiome | 855 | |
| chr1:109,619,434–109,621,357 | 194.6 kb | Distal (>10kb) Multiome | 814 | |
| chr1:109,643,342–109,643,856 | 217.1 kb | Distal (>10kb) Multiome | 470 | |
| chr1:109,655,698–109,656,566 | 229.7 kb | Distal (>10kb) Multiome | 707 | |
| chr1:109,667,812–109,668,362 | 241.6 kb | Distal (>10kb) Multiome | 287 |
Genomic view of the PSMA5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.