The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PSMA3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PSMA3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PSMA3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:58,151,365–58,152,866 | 92.7 kb | Distal (>10kb) Multiome | 506 | |
| chr14:58,199,683–58,200,642 | 44.8 kb | Distal (>10kb) Multiome | 673 | |
| chr14:58,238,784–58,239,254 | 5.6 kb | Proximal (<10kb) | 188 | |
| chr14:58,239,466–58,240,073 | 4.8 kb | Proximal (<10kb) | 480 | |
| chr14:58,244,179–58,245,432 | 31 bp | At TSS Multiome | 789 | |
| chr14:58,297,373–58,299,879 | 53.4 kb | Distal (>10kb) Multiome | 1029 | |
| chr14:58,395,454–58,396,694 | 151.2 kb | Distal (>10kb) Multiome | 791 | |
| chr14:58,426,724–58,428,061 | 182.8 kb | Distal (>10kb) Multiome | 996 |
Genomic view of the PSMA3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.