This gene encodes a member of the peptidase S1 or chymotrypsin family of serine proteases. The encoded preproprotein is proteolytically processed to generate light and heavy chains that associate via a disulfide bond to form the heterodimeric enzyme. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. This protease exhibits trypsin-like substrate specificity, cleaving protein substrates at the carboxyl terminus of lysine or arginine residues. The encoded protease partially mediates proteolytic activation of the epithelial sodium channel, a regulator of sodium balance, and may also play a role in epithelial barrier formation. [provided by RefSeq, Feb 2016]
Transcription factors with Perturb-seq knockdown data for PRSS8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRSS8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRSS8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:30,841,062–30,841,882 | 294.2 kb | Distal (>10kb) Multiome | 49 | |
| chr16:30,874,866–30,875,643 | 260.4 kb | Distal (>10kb) Multiome | 1108 | |
| chr16:30,893,485–30,894,587 | 241.6 kb | Distal (>10kb) Multiome | 950 | |
| chr16:30,895,854–30,897,426 | 238.7 kb | Distal (>10kb) Multiome | 822 | |
| chr16:30,901,969–30,902,794 | 233.4 kb | Distal (>10kb) Multiome | 579 | |
| chr16:30,921,611–30,925,097 | 212.3 kb | Distal (>10kb) Multiome | 1132 | |
| chr16:30,927,705–30,928,596 | 207.6 kb | Distal (>10kb) Multiome | 274 | |
| chr16:30,941,803–30,942,254 | 193.6 kb | Distal (>10kb) Multiome | 507 | |
| chr16:30,947,471–30,950,635 | 185.5 kb | Distal (>10kb) Multiome | 1066 | |
| chr16:30,953,370–30,954,135 | 181.9 kb | Distal (>10kb) Multiome | 499 | |
| chr16:30,956,960–30,958,498 | 178.0 kb | Distal (>10kb) Multiome | 1022 | |
| chr16:30,984,837–30,985,709 | 150.5 kb | Distal (>10kb) Multiome | 787 | |
| chr16:30,996,877–30,998,001 | 138.3 kb | Distal (>10kb) Multiome | 759 | |
| chr16:31,010,379–31,011,032 | 125.0 kb | Distal (>10kb) Multiome | 117 | |
| chr16:31,032,789–31,034,448 | 102.2 kb | Distal (>10kb) Multiome | 1047 | |
| chr16:31,041,996–31,042,665 | 93.4 kb | Distal (>10kb) Multiome | 213 | |
| chr16:31,072,597–31,074,957 | 62.3 kb | Distal (>10kb) Multiome | 1167 | |
| chr16:31,094,132–31,095,139 | 40.9 kb | Distal (>10kb) Multiome | 933 | |
| chr16:31,108,209–31,108,826 | 27.3 kb | Distal (>10kb) Multiome | 733 | |
| chr16:31,117,128–31,118,265 | 18.0 kb | Distal (>10kb) Multiome | 929 | |
| chr16:31,134,682–31,136,040 | 13 bp | At TSS Multiome | 450 | |
| chr16:31,138,695–31,139,457 | 3.4 kb | Proximal (<10kb) Multiome | 413 | |
| chr16:31,141,455–31,143,270 | 6.7 kb | Proximal (<10kb) Multiome | 856 | |
| chr16:31,148,229–31,148,775 | 12.7 kb | Distal (>10kb) Multiome | 209 | |
| chr16:31,179,340–31,181,495 | 44.3 kb | Distal (>10kb) Multiome | 1179 | |
| chr16:31,201,568–31,203,049 | 66.5 kb | Distal (>10kb) Multiome | 507 | |
| chr16:31,214,606–31,216,091 | 79.6 kb | Distal (>10kb) Multiome | 469 | |
| chr16:31,406,131–31,406,947 | 270.8 kb | Distal (>10kb) Multiome | 214 | |
| chr16:31,457,952–31,460,456 | 322.4 kb | Distal (>10kb) Multiome HiCAR | 1128 |
Genomic view of the PRSS8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.