Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]
Transcription factors with Perturb-seq knockdown data for PRKCH. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRKCH upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRKCH, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:61,254,867–61,255,650 | 66.4 kb | Distal (>10kb) Multiome | 219 | |
| chr14:61,280,136–61,281,846 | 40.2 kb | Distal (>10kb) Multiome | 391 | |
| chr14:61,320,928–61,322,807 | 23 bp | At TSS Multiome | 635 | |
| chr14:61,323,085–61,323,272 | 1.5 kb | Proximal (<10kb) | 115 | |
| chr14:61,427,068–61,427,986 | 105.8 kb | Distal (>10kb) Multiome | 9 | |
| chr14:61,567,895–61,569,162 | 247.1 kb | Distal (>10kb) Multiome | 399 | |
| chr14:61,601,551–61,603,283 | 280.4 kb | Distal (>10kb) Multiome | 299 |
Genomic view of the PRKCH locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.