Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
Transcription factors with Perturb-seq knockdown data for PRKCG. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRKCG upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRKCG, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:53,594,477–53,595,909 | 286.9 kb | Distal (>10kb) Multiome | 262 | |
| chr19:53,612,802–53,613,443 | 269.3 kb | Distal (>10kb) Multiome | 182 | |
| chr19:53,792,791–53,793,343 | 89.0 kb | Distal (>10kb) Multiome | 470 | |
| chr19:53,865,880–53,866,753 | 16.0 kb | Distal (>10kb) Multiome | 532 | |
| chr19:53,866,887–53,869,891 | 14.5 kb | Distal (>10kb) Multiome | 993 | |
| chr19:53,878,918–53,880,011 | 2.7 kb | Proximal (<10kb) Multiome | 561 | |
| chr19:53,881,744–53,882,458 | 53 bp | At TSS Multiome | 435 | |
| chr19:53,889,715–53,890,286 | 7.8 kb | Proximal (<10kb) Multiome | 322 | |
| chr19:53,898,176–53,899,412 | 16.8 kb | Distal (>10kb) Multiome | 212 | |
| chr19:53,907,868–53,908,722 | 26.2 kb | Distal (>10kb) Multiome | 276 | |
| chr19:53,941,849–53,943,229 | 60.3 kb | Distal (>10kb) Multiome | 240 | |
| chr19:53,957,309–53,957,858 | 75.3 kb | Distal (>10kb) Multiome | 97 | |
| chr19:53,977,539–53,979,011 | 96.3 kb | Distal (>10kb) Multiome | 412 | |
| chr19:53,979,770–53,982,403 | 98.2 kb | Distal (>10kb) Multiome | 530 | |
| chr19:54,102,597–54,103,302 | 220.6 kb | Distal (>10kb) Multiome | 703 | |
| chr19:54,114,870–54,116,009 | 233.3 kb | Distal (>10kb) Multiome | 911 | |
| chr19:54,136,562–54,138,683 | 255.2 kb | Distal (>10kb) Multiome | 751 | |
| chr19:54,159,030–54,160,865 | 277.7 kb | Distal (>10kb) Multiome | 793 | |
| chr19:54,161,897–54,162,738 | 280.2 kb | Distal (>10kb) Multiome | 233 | |
| chr19:54,164,508–54,165,013 | 282.6 kb | Distal (>10kb) Multiome | 183 | |
| chr19:54,172,536–54,173,532 | 291.0 kb | Distal (>10kb) Multiome | 397 |
Genomic view of the PRKCG locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.