AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2015]
Transcription factors with Perturb-seq knockdown data for PRKAG2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRKAG2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRKAG2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:151,341,187–151,342,118 | 394.6 kb | Distal (>10kb) Multiome | 757 | |
| chr7:151,408,879–151,410,989 | 326.2 kb | Distal (>10kb) Multiome | 407 | |
| chr7:151,439,682–151,441,031 | 296.2 kb | Distal (>10kb) Multiome | 189 | |
| chr7:151,448,171–151,448,932 | 287.6 kb | Distal (>10kb) Multiome | 403 | |
| chr7:151,519,052–151,521,056 | 216.2 kb | Distal (>10kb) Multiome | 899 | |
| chr7:151,626,355–151,626,721 | 5.7 kb | Proximal (<10kb) | 74 | |
| chr7:151,631,518–151,633,118 | 104.0 kb | Distal (>10kb) Multiome | 611 | |
| chr7:151,705,224–151,705,878 | 30.7 kb | Distal (>10kb) Multiome | 192 | |
| chr7:151,782,698–151,783,271 | 46.8 kb | Distal (>10kb) Multiome | 498 | |
| chr7:151,837,433–151,838,118 | 101.5 kb | Distal (>10kb) Multiome | 109 | |
| chr7:151,875,185–151,876,886 | 139.8 kb | Distal (>10kb) Multiome | 561 | |
| chr7:151,877,002–151,877,801 | 141.3 kb | Distal (>10kb) Multiome | 424 | |
| chr7:152,025,196–152,026,407 | 289.4 kb | Distal (>10kb) Multiome | 795 |
Genomic view of the PRKAG2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.