The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PRKAA2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRKAA2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRKAA2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:55,313,810–55,314,345 | 1331.2 kb | Distal (>10kb) Multiome HiCAR | 208 | |
| chr1:55,623,415–55,624,442 | 1021.3 kb | Distal (>10kb) Multiome HiCAR | 86 | |
| chr1:56,396,583–56,397,516 | 248.5 kb | Distal (>10kb) Multiome | 195 | |
| chr1:56,436,904–56,437,573 | 208.1 kb | Distal (>10kb) Multiome | 405 | |
| chr1:56,453,155–56,454,023 | 191.8 kb | Distal (>10kb) Multiome | 234 | |
| chr1:56,477,272–56,478,021 | 167.7 kb | Distal (>10kb) Multiome | 431 | |
| chr1:56,494,285–56,494,744 | 150.8 kb | Distal (>10kb) Multiome | 431 | |
| chr1:56,535,168–56,535,889 | 109.7 kb | Distal (>10kb) Multiome | 257 | |
| chr1:56,550,028–56,550,687 | 94.9 kb | Distal (>10kb) Multiome | 164 | |
| chr1:56,577,020–56,577,756 | 68.0 kb | Distal (>10kb) Multiome | 246 | |
| chr1:56,578,195–56,579,838 | 65.9 kb | Distal (>10kb) Multiome | 784 | |
| chr1:56,644,694–56,646,370 | 41 bp | At TSS Multiome | 601 | |
| chr1:56,811,001–56,811,706 | 166.2 kb | Distal (>10kb) Multiome | 234 | |
| chr1:56,818,824–56,820,020 | 174.2 kb | Distal (>10kb) Multiome | 480 | |
| chr1:56,820,922–56,821,884 | 176.1 kb | Distal (>10kb) Multiome | 419 |
Genomic view of the PRKAA2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.