This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. Alternative splicing of this gene results in multiple transcript variants. This gene has a related pseudogene, which is also present on chromosome 6. [provided by RefSeq, Apr 2014]
Transcription factors with Perturb-seq knockdown data for PRIM2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRIM2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRIM2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:56,953,824–56,956,167 | 362.4 kb | Distal (>10kb) Multiome HiCAR | 903 | |
| chr6:57,046,297–57,047,003 | 271.0 kb | Distal (>10kb) Multiome | 808 | |
| chr6:57,089,489–57,090,518 | 227.6 kb | Distal (>10kb) Multiome | 913 | |
| chr6:57,171,958–57,173,533 | 145.0 kb | Distal (>10kb) Multiome HiCAR | 1049 | |
| chr6:57,221,224–57,222,970 | 95.9 kb | Distal (>10kb) Multiome | 867 | |
| chr6:57,316,348–57,318,082 | 34 bp | At TSS Multiome | 828 | |
| chr6:57,318,958–57,320,128 | 1.9 kb | Proximal (<10kb) Multiome | 130 | |
| chr6:57,423,340–57,424,320 | 106.1 kb | Distal (>10kb) Multiome | 233 |
Genomic view of the PRIM2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.