The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for PRIM1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PRIM1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PRIM1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:56,468,239–56,468,840 | 283.8 kb | Distal (>10kb) Multiome | 907 | |
| chr12:56,487,633–56,488,590 | 264.0 kb | Distal (>10kb) Multiome | 784 | |
| chr12:56,629,725–56,630,674 | 122.0 kb | Distal (>10kb) Multiome | 759 | |
| chr12:56,633,838–56,637,505 | 118.0 kb | Distal (>10kb) Multiome | 1037 | |
| chr12:56,645,530–56,646,466 | 106.2 kb | Distal (>10kb) Multiome | 902 | |
| chr12:56,687,276–56,689,898 | 62.7 kb | Distal (>10kb) Multiome | 1162 | |
| chr12:56,724,695–56,725,713 | 27.0 kb | Distal (>10kb) Multiome | 972 | |
| chr12:56,751,814–56,752,566 | 66 bp | At TSS Multiome | 765 | |
| chr12:57,006,056–57,006,942 | 254.2 kb | Distal (>10kb) Multiome HiCAR | 422 | |
| chr12:57,110,045–57,111,598 | 358.1 kb | Distal (>10kb) Multiome HiCAR | 656 | |
| chr12:57,111,611–57,112,633 | 359.5 kb | Distal (>10kb) Multiome HiCAR | 940 |
Genomic view of the PRIM1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.