Myosin light chain kinase and phosphatase (MLCP) complexes control the phosphorylation states of regulatory myosin light chains, which is crucial for muscle and intracellular movement. MLCPs typically contain a catalytic protein phosphatase 1 (PP1c) subunit, a myosin phosphatase targeting (MYPT) subunit, and another smaller subunit. The protein encoded by this gene represents an MYPT subunit, which is responsible for directing PP1c to its intended targets. However, while the phosphorylation of other MYPT members results in PP1c inactivation, phosphorylation of the encoded protein by protein kinase A results in PP1c activation. [provided by RefSeq, Jan 2020]
Transcription factors with Perturb-seq knockdown data for PPP1R16A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PPP1R16A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PPP1R16A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:144,266,293–144,267,098 | 211.5 kb | Distal (>10kb) Multiome | 162 | |
| chr8:144,290,981–144,292,410 | 186.5 kb | Distal (>10kb) Multiome | 716 | |
| chr8:144,315,833–144,316,450 | 161.8 kb | Distal (>10kb) Multiome | 396 | |
| chr8:144,325,768–144,327,719 | 151.1 kb | Distal (>10kb) Multiome | 1016 | |
| chr8:144,331,281–144,333,847 | 146.0 kb | Distal (>10kb) Multiome | 406 | |
| chr8:144,336,102–144,339,119 | 141.1 kb | Distal (>10kb) Multiome | 922 | |
| chr8:144,353,907–144,355,227 | 123.4 kb | Distal (>10kb) Multiome | 280 | |
| chr8:144,358,142–144,359,070 | 119.4 kb | Distal (>10kb) Multiome | 719 | |
| chr8:144,369,349–144,369,916 | 108.3 kb | Distal (>10kb) Multiome | 204 | |
| chr8:144,372,497–144,372,883 | 105.3 kb | Distal (>10kb) Multiome | 160 | |
| chr8:144,372,993–144,375,084 | 104.0 kb | Distal (>10kb) Multiome | 903 | |
| chr8:144,408,968–144,410,189 | 68.5 kb | Distal (>10kb) Multiome | 920 | |
| chr8:144,427,691–144,429,571 | 49.4 kb | Distal (>10kb) Multiome | 1000 | |
| chr8:144,443,691–144,445,047 | 33.6 kb | Distal (>10kb) Multiome | 743 | |
| chr8:144,462,568–144,463,422 | 15.1 kb | Distal (>10kb) Multiome | 548 | |
| chr8:144,463,885–144,468,178 | 12.2 kb | Distal (>10kb) Multiome | 910 | |
| chr8:144,471,960–144,474,391 | 5.4 kb | Proximal (<10kb) Multiome | 482 | |
| chr8:144,474,517–144,476,543 | 2.1 kb | Proximal (<10kb) Multiome | 321 | |
| chr8:144,476,999–144,477,164 | 816 bp | At TSS | 175 | |
| chr8:144,477,277–144,478,749 | 7 bp | At TSS Multiome | 679 | |
| chr8:144,478,851–144,479,509 | 1.3 kb | Proximal (<10kb) Multiome | 392 | |
| chr8:144,483,979–144,484,843 | 6.4 kb | Proximal (<10kb) Multiome | 125 | |
| chr8:144,495,748–144,496,431 | 18.1 kb | Distal (>10kb) Multiome HiCAR | 131 | |
| chr8:144,500,182–144,501,505 | 23.0 kb | Distal (>10kb) Multiome HiCAR | 484 | |
| chr8:144,507,995–144,510,268 | 30.9 kb | Distal (>10kb) Multiome HiCAR | 1067 | |
| chr8:144,517,168–144,518,442 | 39.9 kb | Distal (>10kb) Multiome HiCAR | 637 | |
| chr8:144,521,935–144,523,450 | 44.5 kb | Distal (>10kb) Multiome HiCAR | 663 | |
| chr8:144,524,105–144,525,099 | 46.7 kb | Distal (>10kb) Multiome HiCAR | 264 | |
| chr8:144,526,399–144,529,305 | 49.1 kb | Distal (>10kb) Multiome HiCAR | 934 | |
| chr8:144,529,455–144,530,630 | 52.2 kb | Distal (>10kb) Multiome HiCAR | 284 | |
| chr8:144,580,290–144,581,657 | 102.8 kb | Distal (>10kb) Multiome | 271 | |
| chr8:144,672,507–144,672,989 | 194.9 kb | Distal (>10kb) Multiome | 150 | |
| chr8:144,684,108–144,686,340 | 206.4 kb | Distal (>10kb) Multiome | 862 | |
| chr8:144,755,078–144,755,956 | 277.6 kb | Distal (>10kb) Multiome | 769 | |
| chr8:144,791,751–144,793,221 | 314.5 kb | Distal (>10kb) Multiome HiCAR | 899 | |
| chr8:144,798,389–144,799,501 | 320.9 kb | Distal (>10kb) Multiome HiCAR | 832 |
Genomic view of the PPP1R16A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.