This gene encodes a protein phosphatase I-interacting protein that promotes the dephosphorylation of eukaryotic translation initiation factor 2A to regulate translation under conditions of cellular stress. The transcribed messenger RNA contains two upstream open reading frames (ORFs) that repress translation of the main protein coding ORF under normal conditions, while the protein coding ORF is expressed at high levels in response to stress. Continual translation of the mRNA under conditions of eukaryotic translation initiation factor 2A inactivation is thought to create a feedback loop for reactivation of the gene during recovery from stress. In addition, it has been shown that this protein plays a role in membrane traffic that is independent of translation and that it is required for exocytosis from erythroleukemia cells. Allelic variants of this gene are associated with microcephaly, short stature, and impaired glucose metabolism. [provided by RefSeq, Feb 2016]
Transcription factors with Perturb-seq knockdown data for PPP1R15B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PPP1R15B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PPP1R15B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:204,128,928–204,129,568 | 282.6 kb | Distal (>10kb) Multiome | 440 | |
| chr1:204,151,279–204,152,703 | 259.8 kb | Distal (>10kb) Multiome | 556 | |
| chr1:204,213,764–204,214,668 | 197.6 kb | Distal (>10kb) Multiome | 702 | |
| chr1:204,287,567–204,288,449 | 123.8 kb | Distal (>10kb) Multiome | 373 | |
| chr1:204,289,294–204,289,779 | 122.3 kb | Distal (>10kb) Multiome | 126 | |
| chr1:204,350,954–204,351,460 | 60.7 kb | Distal (>10kb) Multiome | 138 | |
| chr1:204,363,217–204,363,961 | 48.1 kb | Distal (>10kb) Multiome | 524 | |
| chr1:204,377,771–204,378,537 | 33.7 kb | Distal (>10kb) Multiome | 593 | |
| chr1:204,411,136–204,412,704 | 93 bp | At TSS Multiome | 939 | |
| chr1:204,417,015–204,417,887 | 5.7 kb | Proximal (<10kb) Multiome | 590 | |
| chr1:204,420,137–204,420,371 | 8.2 kb | Proximal (<10kb) | 83 | |
| chr1:204,446,280–204,446,835 | 34.7 kb | Distal (>10kb) Multiome | 311 | |
| chr1:204,493,614–204,495,352 | 82.9 kb | Distal (>10kb) Multiome | 876 | |
| chr1:204,506,146–204,507,240 | 94.7 kb | Distal (>10kb) Multiome | 889 | |
| chr1:204,507,371–204,508,288 | 96.2 kb | Distal (>10kb) Multiome | 508 | |
| chr1:204,515,995–204,516,830 | 104.6 kb | Distal (>10kb) Multiome | 1084 | |
| chr1:204,565,653–204,566,244 | 154.1 kb | Distal (>10kb) Multiome | 403 | |
| chr1:204,684,550–204,685,936 | 273.6 kb | Distal (>10kb) Multiome | 313 | |
| chr1:204,709,678–204,711,587 | 298.7 kb | Distal (>10kb) Multiome | 521 |
Genomic view of the PPP1R15B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.