Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1, catalytic subunit delta), a large regulatory subunit (MYPT, myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2, the first of which is widely expressed, and the second of which may be specific to heart, skeletal muscle, and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome. [provided by RefSeq, Oct 2011]
Transcription factors with Perturb-seq knockdown data for PPP1R12B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PPP1R12B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PPP1R12B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:202,075,813–202,076,531 | 272.5 kb | Distal (>10kb) Multiome | 418 | |
| chr1:202,101,336–202,102,103 | 246.9 kb | Distal (>10kb) Multiome | 173 | |
| chr1:202,105,597–202,106,270 | 242.8 kb | Distal (>10kb) Multiome | 376 | |
| chr1:202,135,239–202,135,691 | 213.3 kb | Distal (>10kb) Multiome | 193 | |
| chr1:202,143,721–202,144,890 | 204.7 kb | Distal (>10kb) Multiome | 589 | |
| chr1:202,184,705–202,185,268 | 163.8 kb | Distal (>10kb) Multiome | 258 | |
| chr1:202,214,010–202,214,579 | 134.3 kb | Distal (>10kb) Multiome | 215 | |
| chr1:202,235,967–202,236,655 | 112.3 kb | Distal (>10kb) Multiome | 460 | |
| chr1:202,237,030–202,237,425 | 111.4 kb | Distal (>10kb) Multiome | 305 | |
| chr1:202,341,446–202,342,246 | 6.7 kb | Proximal (<10kb) Multiome | 728 | |
| chr1:202,348,195–202,349,447 | 57 bp | At TSS Multiome | 898 | |
| chr1:202,638,017–202,638,513 | 289.7 kb | Distal (>10kb) Multiome | 19 | |
| chr1:202,643,050–202,644,080 | 295.2 kb | Distal (>10kb) Multiome | 115 | |
| chr1:202,709,842–202,710,859 | 361.8 kb | Distal (>10kb) Multiome HiCAR | 255 |
Genomic view of the PPP1R12B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.