Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC (EC 4.1.1.36), one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for PPCDC. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PPCDC upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PPCDC, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr15:74,725,773–74,726,537 | 297.4 kb | Distal (>10kb) Multiome | 607 | |
| chr15:74,759,107–74,760,837 | 263.2 kb | Distal (>10kb) Multiome | 637 | |
| chr15:74,761,837–74,762,260 | 261.4 kb | Distal (>10kb) Multiome | 34 | |
| chr15:74,770,975–74,771,578 | 252.3 kb | Distal (>10kb) Multiome | 485 | |
| chr15:74,781,439–74,783,648 | 241.5 kb | Distal (>10kb) Multiome | 679 | |
| chr15:74,842,412–74,843,723 | 180.6 kb | Distal (>10kb) Multiome | 879 | |
| chr15:74,872,771–74,874,092 | 150.3 kb | Distal (>10kb) Multiome | 758 | |
| chr15:74,889,894–74,890,629 | 133.5 kb | Distal (>10kb) Multiome | 816 | |
| chr15:74,905,926–74,907,376 | 116.8 kb | Distal (>10kb) Multiome | 743 | |
| chr15:74,937,494–74,938,710 | 85.5 kb | Distal (>10kb) Multiome | 998 | |
| chr15:74,950,147–74,950,911 | 73.0 kb | Distal (>10kb) Multiome | 326 | |
| chr15:74,954,536–74,955,001 | 68.9 kb | Distal (>10kb) Multiome | 348 | |
| chr15:74,955,873–74,957,720 | 66.8 kb | Distal (>10kb) Multiome | 776 | |
| chr15:74,958,363–74,959,586 | 65.0 kb | Distal (>10kb) Multiome | 282 | |
| chr15:74,995,116–74,996,280 | 28.1 kb | Distal (>10kb) Multiome | 825 | |
| chr15:75,023,365–75,023,895 | 12 bp | At TSS Multiome | 822 | |
| chr15:75,029,350–75,029,565 | 5.8 kb | Proximal (<10kb) | 455 | |
| chr15:75,140,133–75,140,707 | 116.8 kb | Distal (>10kb) Multiome | 536 | |
| chr15:75,178,233–75,178,833 | 154.9 kb | Distal (>10kb) Multiome | 386 | |
| chr15:75,200,527–75,203,502 | 178.4 kb | Distal (>10kb) Multiome HiCAR | 928 |
Genomic view of the PPCDC locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.