PPAT

phosphoribosyl pyrophosphate amidotransferase | GPAT, PRAT

The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]

Member of: DE-6 DE-6.18 Developmental clusters: GC4

Biological processes 12 terms

'de novo' AMP biosynthetic process (GO:0044208)'de novo' IMP biosynthetic process (GO:0006189)'de novo' XMP biosynthetic process (GO:0097294)GMP biosynthetic process (GO:0006177)amidophosphoribosyltransferase activity (GO:0004044)amidophosphoribosyltransferase activity (GO:0004044)amidophosphoribosyltransferase activity (GO:0004044)cytosol (GO:0005829)protein binding (GO:0005515)purine nucleobase biosynthetic process (GO:0009113)purine nucleotide biosynthetic process (GO:0006164)purine nucleotide biosynthetic process (GO:0006164)

Expression (TPM)

TFs regulating PPAT 0 TFs

Transcription factors with Perturb-seq knockdown data for PPAT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PPAT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to PPAT

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PPAT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr4:56,323,870–56,324,663	111.3 kb	Distal (>10kb) Multiome	70
chr4:56,386,710–56,387,993	48.2 kb	Distal (>10kb) Multiome	942
chr4:56,434,804–56,437,263	308 bp	At TSS Multiome	1056
chr4:56,467,264–56,468,300	32.0 kb	Distal (>10kb) Multiome	743
chr4:56,505,059–56,505,907	69.9 kb	Distal (>10kb) Multiome	306
chr4:56,530,151–56,531,207	94.9 kb	Distal (>10kb) Multiome	395
chr4:56,544,602–56,545,190	109.2 kb	Distal (>10kb) Multiome	720
chr4:56,655,262–56,656,591	220.1 kb	Distal (>10kb) Multiome	237

Genome Browser

Genomic view of the PPAT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.