This gene encodes the second largest subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The largest subunit and the encoded protein form the catalytic center of RNA polymerase III. Mutations in this gene are a cause of hypomyelinating leukodystrophy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
Transcription factors with Perturb-seq knockdown data for POLR3B. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = POLR3B upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of POLR3B, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:106,138,295–106,140,144 | 218.6 kb | Distal (>10kb) Multiome | 675 | |
| chr12:106,247,023–106,248,967 | 109.6 kb | Distal (>10kb) Multiome | 593 | |
| chr12:106,301,702–106,303,676 | 54.2 kb | Distal (>10kb) Multiome | 881 | |
| chr12:106,357,226–106,358,686 | 178 bp | At TSS Multiome | 1122 | |
| chr12:106,581,330–106,582,388 | 224.0 kb | Distal (>10kb) Multiome | 219 | |
| chr12:106,582,587–106,584,555 | 225.3 kb | Distal (>10kb) Multiome | 216 | |
| chr12:106,585,673–106,587,837 | 229.6 kb | Distal (>10kb) Multiome | 248 | |
| chr12:106,903,307–106,903,916 | 546.0 kb | Distal (>10kb) Multiome HiCAR | 193 | |
| chr12:106,954,854–106,956,979 | 598.0 kb | Distal (>10kb) Multiome HiCAR | 927 |
Genomic view of the POLR3B locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.