POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]
Transcription factors with Perturb-seq knockdown data for POLE3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = POLE3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of POLE3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:113,150,622–113,151,556 | 259.7 kb | Distal (>10kb) Multiome | 705 | |
| chr9:113,220,980–113,222,241 | 189.2 kb | Distal (>10kb) Multiome | 819 | |
| chr9:113,274,845–113,275,974 | 135.1 kb | Distal (>10kb) Multiome | 852 | |
| chr9:113,331,229–113,332,031 | 78.9 kb | Distal (>10kb) Multiome | 69 | |
| chr9:113,339,770–113,340,691 | 70.4 kb | Distal (>10kb) Multiome | 941 | |
| chr9:113,349,409–113,349,994 | 61.0 kb | Distal (>10kb) Multiome | 358 | |
| chr9:113,376,590–113,377,575 | 33.6 kb | Distal (>10kb) Multiome | 710 | |
| chr9:113,399,812–113,400,390 | 10.0 kb | Proximal (<10kb) | 72 | |
| chr9:113,400,912–113,402,007 | 9.3 kb | Proximal (<10kb) Multiome | 859 | |
| chr9:113,407,014–113,407,193 | 3.2 kb | Proximal (<10kb) | 198 | |
| chr9:113,409,973–113,410,921 | 92 bp | At TSS Multiome | 997 | |
| chr9:113,411,042–113,411,193 | 660 bp | At TSS | 293 | |
| chr9:113,411,295–113,411,475 | 913 bp | At TSS | 65 | |
| chr9:113,463,361–113,464,008 | 53.0 kb | Distal (>10kb) Multiome | 526 | |
| chr9:113,541,084–113,541,543 | 130.7 kb | Distal (>10kb) Multiome | 160 | |
| chr9:113,591,435–113,592,161 | 181.1 kb | Distal (>10kb) Multiome HiCAR | 345 | |
| chr9:113,593,578–113,594,410 | 183.3 kb | Distal (>10kb) Multiome HiCAR | 433 | |
| chr9:113,634,469–113,635,132 | 224.1 kb | Distal (>10kb) Multiome | 142 | |
| chr9:113,658,015–113,658,473 | 247.5 kb | Distal (>10kb) Multiome HiCAR | 307 | |
| chr9:113,692,033–113,692,884 | 281.8 kb | Distal (>10kb) Multiome | 99 |
Genomic view of the POLE3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.