Predicted to be involved in regulation of myoblast fusion. Predicted to act upstream of or within several processes, including lamellipodium morphogenesis; myoblast fusion; and myoblast migration. Predicted to be located in cytoplasm; nucleus; and plasma membrane. Predicted to be active in muscle cell projection membrane and ruffle membrane. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for PLEKHO1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PLEKHO1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PLEKHO1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:149,885,029–149,889,131 | 262.4 kb | Distal (>10kb) Multiome | 1126 | |
| chr1:149,899,425–149,900,227 | 251.0 kb | Distal (>10kb) Multiome | 650 | |
| chr1:149,927,507–149,928,019 | 222.7 kb | Distal (>10kb) Multiome | 744 | |
| chr1:150,010,143–150,011,088 | 139.9 kb | Distal (>10kb) Multiome | 961 | |
| chr1:150,066,903–150,067,886 | 83.0 kb | Distal (>10kb) Multiome | 986 | |
| chr1:150,149,493–150,150,439 | 551 bp | At TSS Multiome | 448 | |
| chr1:150,162,838–150,163,636 | 12.7 kb | Distal (>10kb) Multiome | 550 | |
| chr1:150,213,532–150,214,095 | 63.1 kb | Distal (>10kb) Multiome | 441 | |
| chr1:150,234,435–150,235,519 | 84.1 kb | Distal (>10kb) Multiome | 725 | |
| chr1:150,235,635–150,236,696 | 85.6 kb | Distal (>10kb) Multiome | 905 | |
| chr1:150,255,443–150,256,199 | 105.1 kb | Distal (>10kb) Multiome | 441 | |
| chr1:150,256,563–150,257,324 | 106.4 kb | Distal (>10kb) Multiome | 223 | |
| chr1:150,257,589–150,258,594 | 107.5 kb | Distal (>10kb) Multiome | 233 | |
| chr1:150,259,728–150,260,778 | 109.5 kb | Distal (>10kb) Multiome | 362 | |
| chr1:150,268,574–150,269,453 | 118.5 kb | Distal (>10kb) Multiome | 785 | |
| chr1:150,281,629–150,283,038 | 131.8 kb | Distal (>10kb) Multiome | 503 | |
| chr1:150,293,680–150,294,144 | 143.2 kb | Distal (>10kb) Multiome | 755 | |
| chr1:150,313,761–150,314,506 | 163.5 kb | Distal (>10kb) Multiome | 108 | |
| chr1:150,321,137–150,321,825 | 170.9 kb | Distal (>10kb) Multiome | 806 | |
| chr1:150,363,368–150,364,980 | 213.4 kb | Distal (>10kb) Multiome | 971 |
Genomic view of the PLEKHO1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.