Phosphoinositide 3-kinase gamma is a lipid kinase that produces the lipid second messenger phosphatidylinositol 3,4,5-trisphosphate. The kinase is composed of a catalytic subunit and one of several regulatory subunits, and is chiefly activated by G protein-coupled receptors. This gene encodes a regulatory subunit, and is distantly related to the phosphoinositide-3-kinase, regulatory subunit 5 gene which is located adjacent to this gene on chromosome 7. The orthologous protein in the mouse binds to both the catalytic subunit and to G(beta/gamma), and mediates activation of the kinase subunit downstream of G protein-coupled receptors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Transcription factors with Perturb-seq knockdown data for PIK3R6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PIK3R6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PIK3R6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:8,584,054–8,584,700 | 283.3 kb | Distal (>10kb) Multiome | 18 | |
| chr17:8,629,690–8,631,825 | 236.6 kb | Distal (>10kb) Multiome | 878 | |
| chr17:8,745,813–8,746,432 | 121.6 kb | Distal (>10kb) Multiome | 522 | |
| chr17:8,799,086–8,799,614 | 68.3 kb | Distal (>10kb) Multiome | 168 | |
| chr17:8,874,228–8,874,922 | 6.8 kb | Proximal (<10kb) Multiome | 46 | |
| chr17:8,875,038–8,875,633 | 7.6 kb | Proximal (<10kb) Multiome | 15 | |
| chr17:8,965,232–8,966,153 | 98.1 kb | Distal (>10kb) Multiome | 326 | |
| chr17:9,016,631–9,018,085 | 149.6 kb | Distal (>10kb) Multiome | 402 | |
| chr17:9,020,838–9,022,766 | 153.8 kb | Distal (>10kb) Multiome | 376 | |
| chr17:9,144,444–9,145,038 | 277.0 kb | Distal (>10kb) Multiome | 183 | |
| chr17:9,150,452–9,151,888 | 283.6 kb | Distal (>10kb) Multiome | 267 |
Genomic view of the PIK3R6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.