Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the inositol ring of phosphatidylinositol at the 3-prime position, and play important roles in cell growth, proliferation, differentiation, motility, survival and intracellular trafficking. The PI3Ks are divided into three classes: I, II and III, and only the class I PI3Ks are involved in oncogenesis. This gene encodes the 101 kD regulatory subunit of the class I PI3K gamma complex, which is a dimeric enzyme, consisting of a 110 kD catalytic subunit gamma and a regulatory subunit of either 55, 87 or 101 kD. This protein recruits the catalytic subunit from the cytosol to the plasma membrane through high-affinity interaction with G-beta-gamma proteins. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found. [provided by RefSeq, Oct 2011]
Transcription factors with Perturb-seq knockdown data for PIK3R5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PIK3R5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PIK3R5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:8,745,813–8,746,432 | 219.6 kb | Distal (>10kb) Multiome | 522 | |
| chr17:8,799,086–8,799,614 | 166.3 kb | Distal (>10kb) Multiome | 168 | |
| chr17:8,874,228–8,874,922 | 91.2 kb | Distal (>10kb) Multiome | 46 | |
| chr17:8,875,038–8,875,633 | 90.4 kb | Distal (>10kb) Multiome | 15 | |
| chr17:8,965,232–8,966,153 | 86 bp | At TSS Multiome | 326 | |
| chr17:9,016,631–9,018,085 | 51.5 kb | Distal (>10kb) Multiome | 402 | |
| chr17:9,020,838–9,022,766 | 55.7 kb | Distal (>10kb) Multiome | 376 | |
| chr17:9,144,444–9,145,038 | 179.0 kb | Distal (>10kb) Multiome | 183 | |
| chr17:9,150,452–9,151,888 | 185.5 kb | Distal (>10kb) Multiome | 267 | |
| chr17:9,225,358–9,226,147 | 260.0 kb | Distal (>10kb) Multiome | 198 | |
| chr17:9,239,265–9,241,100 | 274.4 kb | Distal (>10kb) Multiome | 413 | |
| chr17:9,537,200–9,537,700 | 571.7 kb | Distal (>10kb) Multiome HiCAR | 104 | |
| chr17:9,538,516–9,539,118 | 573.2 kb | Distal (>10kb) Multiome HiCAR | 40 |
Genomic view of the PIK3R5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.