PICALM
phosphatidylinositol binding clathrin assembly protein | CALM, CLTH

This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Member of: DE-4 DE-4.3 Developmental clusters: GC7
Biological processes 86 terms
1-phosphatidylinositol binding (GO:0005545)1-phosphatidylinositol binding (GO:0005545)1-phosphatidylinositol binding (GO:0005545)Golgi apparatus (GO:0005794)SNARE binding (GO:0000149)SNARE binding (GO:0000149)amyloid-beta binding (GO:0001540)amyloid-beta clearance by transcytosis (GO:0150093)amyloid-beta clearance by transcytosis (GO:0150093)cadherin binding (GO:0045296)cell surface (GO:0009986)clathrin binding (GO:0030276)clathrin binding (GO:0030276)clathrin coat assembly (GO:0048268)clathrin coat assembly (GO:0048268)clathrin coat assembly (GO:0048268)clathrin coat of coated pit (GO:0030132)clathrin heavy chain binding (GO:0032050)clathrin heavy chain binding (GO:0032050)clathrin-coated endocytic vesicle (GO:0045334)clathrin-coated pit (GO:0005905)clathrin-coated pit (GO:0005905)clathrin-coated pit (GO:0005905)clathrin-coated pit (GO:0005905)clathrin-coated vesicle (GO:0030136)clathrin-coated vesicle (GO:0030136)clathrin-coated vesicle (GO:0030136)clathrin-dependent endocytosis (GO:0072583)clathrin-dependent endocytosis (GO:0072583)clathrin-dependent endocytosis (GO:0072583)cytosol (GO:0005829)early endosome (GO:0005769)endocytosis (GO:0006897)endocytosis (GO:0006897)endomembrane system (GO:0012505)endosomal transport (GO:0016197)endosome to plasma membrane transport vesicle (GO:0070381)extrinsic component of presynaptic endocytic zone membrane (GO:0098894)intracellular iron ion homeostasis (GO:0006879)learning or memory (GO:0007611)low-density lipoprotein particle receptor binding (GO:0050750)membrane (GO:0016020)membrane (GO:0016020)membrane (GO:0016020)membrane bending (GO:0097753)multicellular organismal-level iron ion homeostasis (GO:0060586)negative regulation of gene expression (GO:0010629)negative regulation of protein localization to cell surface (GO:2000009)negative regulation of protein localization to plasma membrane (GO:1903077)negative regulation of receptor-mediated endocytosis (GO:0048261)neurofibrillary tangle (GO:0097418)neuronal cell body (GO:0043025)nucleus (GO:0005634)nucleus (GO:0005634)perinuclear region of cytoplasm (GO:0048471)phosphatidylinositol-4,5-bisphosphate binding (GO:0005546)phosphatidylinositol-4,5-bisphosphate binding (GO:0005546)phospholipid binding (GO:0005543)plasma membrane (GO:0005886)plasma membrane (GO:0005886)positive regulation of DNA-templated transcription (GO:0045893)positive regulation of Ras protein signal transduction (GO:0046579)positive regulation of amyloid precursor protein catabolic process (GO:1902993)positive regulation of amyloid-beta formation (GO:1902004)postsynaptic membrane (GO:0045211)presynaptic membrane (GO:0042734)protein binding (GO:0005515)protein-containing complex assembly (GO:0065003)receptor internalization (GO:0031623)receptor-mediated endocytosis (GO:0006898)regulation of amyloid precursor protein catabolic process (GO:1902991)regulation of amyloid-beta formation (GO:1902003)regulation of endocytosis (GO:0030100)regulation of protein localization (GO:0032880)regulation of receptor-mediated endocytosis (GO:0048259)regulation of vesicle size (GO:0097494)small GTPase binding (GO:0031267)synaptic vesicle (GO:0008021)synaptic vesicle maturation (GO:0016188)tau protein binding (GO:0048156)vesicle (GO:0031982)vesicle (GO:0031982)vesicle budding from membrane (GO:0006900)vesicle budding from membrane (GO:0006900)vesicle cargo loading (GO:0035459)vesicle-mediated transport (GO:0016192)
Expression (TPM)
PICALM — as a Regulated Gene

TFs regulating PICALM 0 TFs

Transcription factors with Perturb-seq knockdown data for PICALM. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PICALM upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to PICALM

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PICALM, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr11:85,809,696–85,811,885 257.9 kb Distal (>10kb) Multiome 535
chr11:85,854,605–85,855,812 214.0 kb Distal (>10kb) Multiome 948
chr11:86,052,796–86,053,429 16.0 kb Distal (>10kb) Multiome 169
chr11:86,068,044–86,070,093 128 bp At TSS Multiome 1026
chr11:86,151,187–86,151,901 82.4 kb Distal (>10kb) Multiome 356
chr11:86,172,744–86,173,502 103.9 kb Distal (>10kb) Multiome HiCAR 240
chr11:86,244,135–86,246,099 175.9 kb Distal (>10kb) Multiome 959
chr11:86,302,015–86,302,729 233.2 kb Distal (>10kb) Multiome 873

Genome Browser

Genomic view of the PICALM locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr11:85,799,696 – 86,312,729
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq