The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. This gene product is specifically expressed in the brain, however, its exact function is not known. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2010]
Transcription factors with Perturb-seq knockdown data for PGBD1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PGBD1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PGBD1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:28,079,998–28,081,441 | 201.4 kb | Distal (>10kb) Multiome | 1054 | |
| chr6:28,104,510–28,105,291 | 176.7 kb | Distal (>10kb) Multiome | 927 | |
| chr6:28,136,684–28,137,470 | 144.5 kb | Distal (>10kb) Multiome | 1035 | |
| chr6:28,141,479–28,142,195 | 139.7 kb | Distal (>10kb) Multiome | 1010 | |
| chr6:28,171,036–28,171,660 | 110.1 kb | Distal (>10kb) Multiome | 67 | |
| chr6:28,225,159–28,225,667 | 56.2 kb | Distal (>10kb) Multiome | 914 | |
| chr6:28,281,346–28,282,033 | 3 bp | At TSS Multiome | 751 | |
| chr6:28,335,073–28,336,704 | 54.2 kb | Distal (>10kb) Multiome | 1036 | |
| chr6:28,349,817–28,350,289 | 68.5 kb | Distal (>10kb) Multiome | 884 | |
| chr6:28,356,177–28,356,613 | 74.8 kb | Distal (>10kb) Multiome | 569 | |
| chr6:28,383,266–28,383,869 | 102.0 kb | Distal (>10kb) Multiome | 821 | |
| chr6:28,399,292–28,400,183 | 118.2 kb | Distal (>10kb) Multiome | 978 | |
| chr6:28,443,132–28,443,643 | 161.9 kb | Distal (>10kb) Multiome | 481 | |
| chr6:28,468,726–28,469,991 | 187.7 kb | Distal (>10kb) Multiome | 36 | |
| chr6:28,542,393–28,543,473 | 261.2 kb | Distal (>10kb) Multiome | 775 |
Genomic view of the PGBD1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.