This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. The N-terminal TRX-like domain is the primary binding site for the major ER chaperone calreticulin and possibly other proteins and substrates as well. Alternative splicing results in multiple protein- and non-protein-coding transcript variants. [provided by RefSeq, Dec 2016]
Transcription factors with Perturb-seq knockdown data for PDIA5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = PDIA5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of PDIA5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:122,798,643–122,799,856 | 267.8 kb | Distal (>10kb) Multiome | 131 | |
| chr3:122,890,181–122,890,667 | 176.7 kb | Distal (>10kb) Multiome | 154 | |
| chr3:122,942,761–122,943,436 | 123.9 kb | Distal (>10kb) Multiome | 336 | |
| chr3:122,975,104–122,975,992 | 91.5 kb | Distal (>10kb) Multiome | 338 | |
| chr3:123,026,031–123,028,771 | 39.2 kb | Distal (>10kb) Multiome | 478 | |
| chr3:123,066,643–123,067,789 | 23 bp | At TSS Multiome | 865 | |
| chr3:123,068,200–123,069,312 | 1.2 kb | Proximal (<10kb) | 332 | |
| chr3:123,080,786–123,081,476 | 14.0 kb | Distal (>10kb) Multiome | 291 | |
| chr3:123,081,598–123,082,265 | 15.1 kb | Distal (>10kb) Multiome | 186 | |
| chr3:123,143,533–123,144,461 | 77.1 kb | Distal (>10kb) Multiome HiCAR | 294 | |
| chr3:123,169,519–123,169,980 | 102.7 kb | Distal (>10kb) Multiome | 64 | |
| chr3:123,201,446–123,202,796 | 134.8 kb | Distal (>10kb) Multiome | 651 | |
| chr3:123,314,223–123,315,571 | 248.0 kb | Distal (>10kb) Multiome | 264 |
Genomic view of the PDIA5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.