The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis. [provided by RefSeq, Oct 2010]
Transcription factors with Perturb-seq knockdown data for ORC6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ORC6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ORC6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:46,620,934–46,621,754 | 68.3 kb | Distal (>10kb) Multiome | 816 | |
| chr16:46,632,606–46,634,034 | 56.3 kb | Distal (>10kb) Multiome | 427 | |
| chr16:46,688,335–46,690,548 | 331 bp | At TSS Multiome | 1034 | |
| chr16:46,690,771–46,691,129 | 1.1 kb | Proximal (<10kb) | 246 | |
| chr16:46,739,861–46,740,427 | 50.6 kb | Distal (>10kb) Multiome | 134 | |
| chr16:46,789,580–46,790,544 | 100.5 kb | Distal (>10kb) Multiome | 725 | |
| chr16:46,830,588–46,832,033 | 141.7 kb | Distal (>10kb) Multiome | 792 | |
| chr16:46,883,418–46,885,208 | 194.6 kb | Distal (>10kb) Multiome | 969 | |
| chr16:46,885,524–46,886,746 | 196.7 kb | Distal (>10kb) Multiome | 318 | |
| chr16:46,972,819–46,974,091 | 284.1 kb | Distal (>10kb) Multiome | 975 |
Genomic view of the ORC6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.