The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate. [provided by RefSeq, Oct 2010]
Transcription factors with Perturb-seq knockdown data for ORC2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ORC2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ORC2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:200,713,048–200,713,472 | 250.4 kb | Distal (>10kb) Multiome | 763 | |
| chr2:200,736,302–200,737,549 | 226.7 kb | Distal (>10kb) Multiome | 210 | |
| chr2:200,810,854–200,812,782 | 152.1 kb | Distal (>10kb) Multiome | 1048 | |
| chr2:200,863,766–200,865,261 | 99.0 kb | Distal (>10kb) Multiome | 1111 | |
| chr2:200,888,532–200,889,950 | 74.3 kb | Distal (>10kb) Multiome | 1108 | |
| chr2:200,962,915–200,964,242 | 53 bp | At TSS Multiome | 1021 | |
| chr2:201,071,202–201,072,338 | 108.1 kb | Distal (>10kb) Multiome | 993 | |
| chr2:201,090,426–201,091,071 | 127.1 kb | Distal (>10kb) Multiome | 68 | |
| chr2:201,116,279–201,117,190 | 153.0 kb | Distal (>10kb) Multiome | 907 | |
| chr2:201,118,264–201,119,153 | 155.1 kb | Distal (>10kb) Multiome | 863 | |
| chr2:201,125,205–201,126,756 | 162.7 kb | Distal (>10kb) Multiome | 696 | |
| chr2:201,157,926–201,158,657 | 194.7 kb | Distal (>10kb) Multiome HiCAR | 540 | |
| chr2:201,182,711–201,183,380 | 219.4 kb | Distal (>10kb) Multiome HiCAR | 624 |
Genomic view of the ORC2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.