The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers. [provided by RefSeq, Oct 2010]
Transcription factors with Perturb-seq knockdown data for ODF2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ODF2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ODF2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:128,159,371–128,160,928 | 296.0 kb | Distal (>10kb) Multiome | 824 | |
| chr9:128,191,300–128,192,050 | 264.5 kb | Distal (>10kb) Multiome | 865 | |
| chr9:128,203,004–128,204,704 | 251.8 kb | Distal (>10kb) Multiome | 690 | |
| chr9:128,218,656–128,219,107 | 237.3 kb | Distal (>10kb) Multiome | 410 | |
| chr9:128,250,012–128,251,099 | 205.6 kb | Distal (>10kb) Multiome | 353 | |
| chr9:128,275,636–128,276,535 | 180.1 kb | Distal (>10kb) Multiome | 728 | |
| chr9:128,322,183–128,322,933 | 133.5 kb | Distal (>10kb) Multiome | 962 | |
| chr9:128,340,070–128,341,161 | 115.7 kb | Distal (>10kb) Multiome | 803 | |
| chr9:128,364,858–128,365,437 | 91.0 kb | Distal (>10kb) Multiome | 442 | |
| chr9:128,420,259–128,421,260 | 35.4 kb | Distal (>10kb) Multiome | 701 | |
| chr9:128,455,721–128,456,522 | 83 bp | At TSS Multiome | 684 | |
| chr9:128,456,806–128,457,003 | 655 bp | At TSS | 340 | |
| chr9:128,504,482–128,505,188 | 48.6 kb | Distal (>10kb) Multiome | 766 | |
| chr9:128,552,006–128,553,125 | 96.4 kb | Distal (>10kb) Multiome | 839 | |
| chr9:128,656,436–128,657,126 | 200.7 kb | Distal (>10kb) Multiome | 1009 | |
| chr9:128,683,393–128,684,010 | 227.5 kb | Distal (>10kb) Multiome | 296 | |
| chr9:128,684,170–128,684,813 | 228.6 kb | Distal (>10kb) Multiome | 574 | |
| chr9:128,688,449–128,689,713 | 232.9 kb | Distal (>10kb) Multiome | 805 | |
| chr9:128,702,246–128,702,946 | 246.4 kb | Distal (>10kb) Multiome | 720 | |
| chr9:128,723,691–128,725,229 | 268.1 kb | Distal (>10kb) Multiome | 821 |
Genomic view of the ODF2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.