This gene encodes a nuclear protein that is highly conserved in vertebrates. The conserved regions of the protein contain several consensus phosphorylation sites for casein kinase II and cyclin-dependent kinases, two putative nuclear localization signals, and a basic DNA-binding domain. It is phosphorylated in vivo by Cdk1 during mitosis of the cell cycle. [provided by RefSeq, Aug 2010]
Transcription factors with Perturb-seq knockdown data for NUCKS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NUCKS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NUCKS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:205,449,573–205,450,965 | 300.1 kb | Distal (>10kb) Multiome | 512 | |
| chr1:205,456,289–205,457,681 | 293.0 kb | Distal (>10kb) Multiome | 383 | |
| chr1:205,485,282–205,485,813 | 264.6 kb | Distal (>10kb) Multiome | 590 | |
| chr1:205,502,456–205,503,022 | 247.5 kb | Distal (>10kb) Multiome | 45 | |
| chr1:205,504,221–205,505,625 | 245.5 kb | Distal (>10kb) Multiome | 631 | |
| chr1:205,528,647–205,529,482 | 221.0 kb | Distal (>10kb) Multiome | 390 | |
| chr1:205,543,280–205,544,028 | 206.6 kb | Distal (>10kb) Multiome | 371 | |
| chr1:205,591,729–205,592,393 | 158.1 kb | Distal (>10kb) Multiome | 624 | |
| chr1:205,631,034–205,632,587 | 118.3 kb | Distal (>10kb) Multiome | 1054 | |
| chr1:205,670,539–205,671,380 | 79.3 kb | Distal (>10kb) Multiome | 189 | |
| chr1:205,679,697–205,680,913 | 69.7 kb | Distal (>10kb) Multiome | 568 | |
| chr1:205,706,859–205,707,298 | 43.3 kb | Distal (>10kb) Multiome | 145 | |
| chr1:205,748,908–205,750,848 | 634 bp | At TSS Multiome | 1074 | |
| chr1:205,774,834–205,775,616 | 25.2 kb | Distal (>10kb) Multiome | 637 | |
| chr1:205,812,462–205,814,100 | 63.2 kb | Distal (>10kb) Multiome | 693 | |
| chr1:205,849,914–205,850,368 | 100.0 kb | Distal (>10kb) Multiome | 143 | |
| chr1:205,918,766–205,919,275 | 168.9 kb | Distal (>10kb) Multiome HiCAR | 284 |
Genomic view of the NUCKS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.