This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]
Transcription factors with Perturb-seq knockdown data for NSMAF. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NSMAF upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NSMAF, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:58,410,831–58,412,003 | 248.3 kb | Distal (>10kb) Multiome | 778 | |
| chr8:58,552,612–58,554,201 | 106.5 kb | Distal (>10kb) Multiome | 932 | |
| chr8:58,658,535–58,660,031 | 127 bp | At TSS Multiome | 881 | |
| chr8:58,671,394–58,672,805 | 12.6 kb | Distal (>10kb) Multiome | 324 | |
| chr8:60,651,471–60,652,894 | 1992.5 kb | Distal (>10kb) Multiome HiCAR | 899 | |
| chr8:60,653,001–60,654,152 | 1994.0 kb | Distal (>10kb) Multiome HiCAR | 520 |
Genomic view of the NSMAF locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.