This gene encodes a member of the N-acetylneuraminate lyase sub-family of (beta/alpha)(8)-barrel enzymes. N-acetylneuraminate lyases regulate cellular concentrations of N-acetyl-neuraminic acid (sialic acid) by mediating the reversible conversion of sialic acid into N-acetylmannosamine and pyruvate. A pseudogene of this gene is located on the short arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
Transcription factors with Perturb-seq knockdown data for NPL. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NPL upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NPL, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:182,084,244–182,085,384 | 704.7 kb | Distal (>10kb) Multiome HiCAR | 393 | |
| chr1:182,101,254–182,101,977 | 688.1 kb | Distal (>10kb) Multiome HiCAR | 357 | |
| chr1:182,390,343–182,392,611 | 397.9 kb | Distal (>10kb) Multiome HiCAR | 1089 | |
| chr1:182,603,748–182,604,847 | 185.4 kb | Distal (>10kb) Multiome HiCAR | 617 | |
| chr1:182,611,551–182,612,259 | 177.8 kb | Distal (>10kb) Multiome HiCAR | 222 | |
| chr1:182,614,397–182,617,189 | 174.8 kb | Distal (>10kb) Multiome HiCAR | 882 | |
| chr1:182,699,357–182,700,582 | 89.7 kb | Distal (>10kb) Multiome HiCAR | 237 | |
| chr1:182,781,932–182,782,627 | 7.1 kb | Proximal (<10kb) | 334 | |
| chr1:182,786,771–182,786,994 | 2.8 kb | Proximal (<10kb) | 112 | |
| chr1:182,789,198–182,790,098 | 113 bp | At TSS Multiome | 771 | |
| chr1:182,791,259–182,791,628 | 1.5 kb | Proximal (<10kb) | 123 | |
| chr1:182,791,741–182,792,085 | 2.0 kb | Proximal (<10kb) | 230 | |
| chr1:182,806,097–182,806,606 | 16.6 kb | Distal (>10kb) Multiome | 157 | |
| chr1:182,838,565–182,840,149 | 49.6 kb | Distal (>10kb) Multiome | 1015 | |
| chr1:182,957,895–182,958,423 | 168.3 kb | Distal (>10kb) Multiome | 75 | |
| chr1:183,022,283–183,024,397 | 233.5 kb | Distal (>10kb) Multiome | 950 |
Genomic view of the NPL locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.