The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for NOL6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NOL6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NOL6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:33,264,068–33,265,475 | 209.1 kb | Distal (>10kb) Multiome | 790 | |
| chr9:33,290,056–33,291,152 | 183.4 kb | Distal (>10kb) Multiome | 1075 | |
| chr9:33,381,203–33,381,714 | 92.4 kb | Distal (>10kb) Multiome | 134 | |
| chr9:33,447,056–33,448,058 | 26.3 kb | Distal (>10kb) Multiome | 547 | |
| chr9:33,452,742–33,453,295 | 20.9 kb | Distal (>10kb) Multiome | 374 | |
| chr9:33,466,696–33,466,937 | 7.0 kb | Proximal (<10kb) | 268 | |
| chr9:33,473,593–33,474,250 | 81 bp | At TSS Multiome | 635 | |
| chr9:33,474,436–33,474,720 | 534 bp | At TSS | 156 | |
| chr9:33,523,393–33,524,316 | 49.9 kb | Distal (>10kb) Multiome | 303 | |
| chr9:33,676,675–33,677,521 | 203.2 kb | Distal (>10kb) Multiome | 242 | |
| chr9:33,721,959–33,722,424 | 248.2 kb | Distal (>10kb) Multiome | 461 |
Genomic view of the NOL6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.