In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al. (2004) [PubMed 15056733].[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for NKX6-1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NKX6-1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NKX6-1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:84,492,414–84,493,844 | 3.1 kb | Proximal (<10kb) | 257 | |
| chr4:84,495,362–84,495,799 | 1.1 kb | Proximal (<10kb) | 60 | |
| chr4:84,496,083–84,500,043 | at TSS | At TSS | 567 | |
| chr4:84,501,767–84,502,114 | 4.8 kb | Proximal (<10kb) | 54 | |
| chr4:84,503,937–84,504,153 | 7.0 kb | Proximal (<10kb) | 150 |
Genomic view of the NKX6-1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.