NIT1

nitrilase 1

This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Member of: DE-1

Biological processes 12 terms

cytoplasm (GO:0005737)cytoplasm (GO:0005737)deaminated glutathione amidase activity (GO:0110050)deaminated glutathione amidase activity (GO:0110050)deaminated glutathione amidase activity (GO:0110050)glutathione metabolic process (GO:0006749)hydrolase activity (GO:0016787)hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides (GO:0016811)mitochondrion (GO:0005739)mitochondrion (GO:0005739)mitochondrion (GO:0005739)nucleus (GO:0005634)

Expression (TPM)

TFs regulating NIT1 0 TFs

Transcription factors with Perturb-seq knockdown data for NIT1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NIT1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to NIT1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NIT1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr1:160,681,274–160,681,823	436.5 kb	Distal (>10kb) Multiome HiCAR	237
chr1:160,910,331–160,911,177	207.4 kb	Distal (>10kb) Multiome	97
chr1:160,947,055–160,947,669	170.7 kb	Distal (>10kb) Multiome	35
chr1:160,948,561–160,949,851	168.7 kb	Distal (>10kb) Multiome	173
chr1:160,981,323–160,982,552	136.3 kb	Distal (>10kb) Multiome	156
chr1:161,020,536–161,021,920	96.9 kb	Distal (>10kb) Multiome	846
chr1:161,038,489–161,039,130	79.2 kb	Distal (>10kb) Multiome	468
chr1:161,045,080–161,046,516	72.1 kb	Distal (>10kb) Multiome	927
chr1:161,097,691–161,099,047	19.9 kb	Distal (>10kb) Multiome	829
chr1:161,117,504–161,118,419	44 bp	At TSS Multiome	794
chr1:161,119,718–161,119,933	1.6 kb	Proximal (<10kb)	155
chr1:161,131,760–161,133,351	14.6 kb	Distal (>10kb) Multiome	956
chr1:161,153,700–161,154,461	35.9 kb	Distal (>10kb) Multiome	816
chr1:161,158,928–161,160,167	41.4 kb	Distal (>10kb) Multiome	706
chr1:161,165,740–161,167,170	48.2 kb	Distal (>10kb) Multiome	888
chr1:161,176,874–161,177,972	59.4 kb	Distal (>10kb) Multiome	910
chr1:161,198,215–161,199,209	80.4 kb	Distal (>10kb) Multiome	357
chr1:161,201,572–161,202,783	84.2 kb	Distal (>10kb) Multiome	852
chr1:161,225,326–161,226,910	107.8 kb	Distal (>10kb) Multiome	710
chr1:161,227,235–161,227,802	109.4 kb	Distal (>10kb) Multiome	372
chr1:161,258,515–161,259,098	140.7 kb	Distal (>10kb) Multiome	281
chr1:161,305,737–161,306,708	188.2 kb	Distal (>10kb) Multiome	430
chr1:161,313,977–161,314,703	196.2 kb	Distal (>10kb) Multiome	824
chr1:161,389,675–161,390,784	272.0 kb	Distal (>10kb) Multiome	866
chr1:161,399,098–161,400,259	281.5 kb	Distal (>10kb) Multiome	1040

Genome Browser

Genomic view of the NIT1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.