Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for NHEJ1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NHEJ1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NHEJ1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:218,860,491–218,861,382 | 299.8 kb | Distal (>10kb) Multiome | 241 | |
| chr2:218,883,560–218,884,239 | 276.9 kb | Distal (>10kb) Multiome | 190 | |
| chr2:218,892,714–218,893,400 | 267.9 kb | Distal (>10kb) Multiome | 591 | |
| chr2:218,898,077–218,898,730 | 262.5 kb | Distal (>10kb) Multiome | 624 | |
| chr2:218,908,757–218,909,543 | 251.7 kb | Distal (>10kb) Multiome | 885 | |
| chr2:218,959,145–218,960,352 | 201.3 kb | Distal (>10kb) Multiome | 416 | |
| chr2:218,962,902–218,964,278 | 197.6 kb | Distal (>10kb) Multiome | 768 | |
| chr2:218,979,814–218,980,492 | 180.7 kb | Distal (>10kb) Multiome | 499 | |
| chr2:218,984,382–218,985,013 | 176.2 kb | Distal (>10kb) Multiome | 126 | |
| chr2:218,992,930–218,994,312 | 167.3 kb | Distal (>10kb) Multiome | 265 | |
| chr2:218,995,885–218,998,018 | 164.6 kb | Distal (>10kb) Multiome | 534 | |
| chr2:218,998,611–219,003,562 | 158.8 kb | Distal (>10kb) Multiome | 941 | |
| chr2:219,057,853–219,058,462 | 102.7 kb | Distal (>10kb) Multiome | 469 | |
| chr2:219,060,260–219,061,256 | 100.0 kb | Distal (>10kb) Multiome | 240 | |
| chr2:219,159,910–219,161,297 | 288 bp | At TSS Multiome | 901 | |
| chr2:219,176,168–219,177,450 | 16.0 kb | Distal (>10kb) Multiome | 999 | |
| chr2:219,177,596–219,178,930 | 17.4 kb | Distal (>10kb) Multiome | 930 | |
| chr2:219,206,194–219,207,456 | 46.0 kb | Distal (>10kb) Multiome | 861 | |
| chr2:219,217,908–219,219,751 | 58.1 kb | Distal (>10kb) Multiome | 1058 | |
| chr2:219,228,940–219,230,470 | 68.8 kb | Distal (>10kb) Multiome | 914 | |
| chr2:219,245,074–219,245,804 | 84.6 kb | Distal (>10kb) Multiome | 891 | |
| chr2:219,252,908–219,254,656 | 93.1 kb | Distal (>10kb) Multiome | 808 | |
| chr2:219,277,516–219,280,537 | 118.5 kb | Distal (>10kb) Multiome | 1073 | |
| chr2:219,294,524–219,295,391 | 134.1 kb | Distal (>10kb) Multiome HiCAR | 892 | |
| chr2:219,308,224–219,309,089 | 147.5 kb | Distal (>10kb) Multiome | 742 | |
| chr2:219,386,994–219,389,117 | 227.4 kb | Distal (>10kb) Multiome | 911 | |
| chr2:219,402,761–219,403,203 | 242.1 kb | Distal (>10kb) Multiome | 234 | |
| chr2:219,418,154–219,419,089 | 257.9 kb | Distal (>10kb) Multiome | 147 | |
| chr2:219,433,920–219,435,459 | 273.3 kb | Distal (>10kb) Multiome | 315 | |
| chr2:219,441,439–219,442,557 | 281.1 kb | Distal (>10kb) Multiome | 338 | |
| chr2:219,447,946–219,449,531 | 288.0 kb | Distal (>10kb) Multiome | 244 |
Genomic view of the NHEJ1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.