The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for NFYA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NFYA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NFYA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:40,790,904–40,791,637 | 281.7 kb | Distal (>10kb) Multiome | 134 | |
| chr6:40,996,645–40,997,964 | 75.3 kb | Distal (>10kb) Multiome | 624 | |
| chr6:41,027,596–41,028,853 | 44.8 kb | Distal (>10kb) Multiome | 786 | |
| chr6:41,071,799–41,073,997 | 162 bp | At TSS Multiome | 1135 | |
| chr6:41,100,767–41,101,294 | 28.0 kb | Distal (>10kb) Multiome | 204 | |
| chr6:41,171,808–41,172,283 | 99.2 kb | Distal (>10kb) Multiome | 156 | |
| chr6:41,318,625–41,319,323 | 246.0 kb | Distal (>10kb) Multiome | 629 |
Genomic view of the NFYA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.