NFKB1

NFKB1

nuclear factor kappa B subunit 1 | KBF1, NF-kB1, NF-kappaB, NFKB-p50, NFkappaB, p105, p50

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. NFKB is a critical regulator of the immediate-early response to viral infection. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Aug 2020]

Member of: DE-5

Biological processes 101 terms

B cell receptor signaling pathway (GO:0050853)DNA binding (GO:0003677)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription repressor activity, RNA polymerase II-specific (GO:0001227)I-kappaB/NF-kappaB complex (GO:0033256)I-kappaB/NF-kappaB complex (GO:0033256)JNK cascade (GO:0007254)MAPK cascade (GO:0000165)NF-kappaB p50/p65 complex (GO:0035525)NF-kappaB p50/p65 complex (GO:0035525)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)actinin binding (GO:0042805)antibacterial innate immune response (GO:0140367)canonical NF-kappaB signal transduction (GO:0007249)canonical NF-kappaB signal transduction (GO:0007249)canonical NF-kappaB signal transduction (GO:0007249)cellular response to angiotensin (GO:1904385)cellular response to interleukin-17 (GO:0097398)cellular response to interleukin-6 (GO:0071354)cellular response to lipopolysaccharide (GO:0071222)cellular response to lipopolysaccharide (GO:0071222)cellular response to mechanical stimulus (GO:0071260)cellular response to nicotine (GO:0071316)cellular response to tumor necrosis factor (GO:0071356)cellular response to virus (GO:0098586)chromatin (GO:0000785)chromatin (GO:0000785)chromatin (GO:0000785)chromatin binding (GO:0003682)cis-regulatory region sequence-specific DNA binding (GO:0000987)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)cytosol (GO:0005829)cytosol (GO:0005829)extracellular region (GO:0005576)gene expression (GO:0010467)identical protein binding (GO:0042802)identical protein binding (GO:0042802)inflammatory response (GO:0006954)mammary gland involution (GO:0060056)negative regulation of apoptotic process (GO:0043066)negative regulation of cytokine production (GO:0001818)negative regulation of cytokine production involved in inflammatory response (GO:1900016)negative regulation of gene expression (GO:0010629)negative regulation of inflammatory response (GO:0050728)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of transcription by RNA polymerase II (GO:0000122)negative regulation of vitamin D biosynthetic process (GO:0010957)non-canonical NF-kappaB signal transduction (GO:0038061)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of DNA-templated transcription (GO:0045893)positive regulation of DNA-templated transcription (GO:0045893)positive regulation of DNA-templated transcription (GO:0045893)positive regulation of canonical Wnt signaling pathway (GO:0090263)positive regulation of cholesterol efflux (GO:0010875)positive regulation of hyaluronan biosynthetic process (GO:1900127)positive regulation of lipid storage (GO:0010884)positive regulation of macrophage derived foam cell differentiation (GO:0010744)positive regulation of miRNA metabolic process (GO:2000630)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription initiation by RNA polymerase II (GO:0060261)protein binding (GO:0005515)protein sequestering activity (GO:0140311)regulation of DNA-templated transcription (GO:0006355)regulation of gene expression (GO:0010468)regulation of transcription by RNA polymerase II (GO:0006357)secretory granule lumen (GO:0034774)sequence-specific DNA binding (GO:0043565)signal transduction (GO:0007165)specific granule lumen (GO:0035580)transcription by RNA polymerase II (GO:0006366)transcription cis-regulatory region binding (GO:0000976)transcription coactivator binding (GO:0001223)transcription coregulator activity (GO:0003712)tumor necrosis factor-mediated signaling pathway (GO:0033209)

Expression (TPM)

TFs regulating NFKB1 0 TFs

Transcription factors with Perturb-seq knockdown data for NFKB1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NFKB1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to NFKB1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NFKB1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility	Element	Dist. to TSS	Link type	TFs
	chr4:102,319,212–102,320,155	182.1 kb	Distal (>10kb) Multiome	159
	chr4:102,343,901–102,346,340	156.4 kb	Distal (>10kb) Multiome	949
	chr4:102,430,899–102,431,874	70.6 kb	Distal (>10kb) Multiome	452
	chr4:102,492,717–102,493,103	8.5 kb	Proximal (<10kb)	33
	chr4:102,493,204–102,493,987	7.6 kb	Proximal (<10kb)	194
	chr4:102,500,438–102,502,654	265 bp	At TSS Multiome	906
	chr4:102,759,924–102,761,752	259.1 kb	Distal (>10kb) Multiome	907

Genome Browser

Genomic view of the NFKB1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.