NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for NELFB. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NELFB upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NELFB, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:136,977,106–136,980,430 | 276.8 kb | Distal (>10kb) Multiome | 413 | |
| chr9:136,991,827–136,993,248 | 263.0 kb | Distal (>10kb) Multiome | 720 | |
| chr9:137,027,536–137,029,054 | 227.1 kb | Distal (>10kb) Multiome | 539 | |
| chr9:137,029,231–137,029,652 | 225.8 kb | Distal (>10kb) Multiome | 386 | |
| chr9:137,045,334–137,046,838 | 208.8 kb | Distal (>10kb) Multiome | 829 | |
| chr9:137,053,352–137,054,892 | 201.2 kb | Distal (>10kb) Multiome | 544 | |
| chr9:137,067,810–137,068,494 | 187.2 kb | Distal (>10kb) Multiome | 492 | |
| chr9:137,069,879–137,071,138 | 184.7 kb | Distal (>10kb) Multiome | 703 | |
| chr9:137,076,758–137,078,723 | 177.9 kb | Distal (>10kb) Multiome | 530 | |
| chr9:137,085,844–137,087,716 | 168.5 kb | Distal (>10kb) Multiome | 890 | |
| chr9:137,129,244–137,130,499 | 125.5 kb | Distal (>10kb) Multiome | 823 | |
| chr9:137,167,984–137,170,155 | 85.8 kb | Distal (>10kb) Multiome | 560 | |
| chr9:137,188,099–137,189,538 | 66.6 kb | Distal (>10kb) Multiome | 954 | |
| chr9:137,200,175–137,201,689 | 54.4 kb | Distal (>10kb) Multiome | 856 | |
| chr9:137,205,371–137,205,975 | 49.7 kb | Distal (>10kb) Multiome | 758 | |
| chr9:137,221,373–137,224,294 | 33.4 kb | Distal (>10kb) Multiome | 843 | |
| chr9:137,227,249–137,228,051 | 27.6 kb | Distal (>10kb) Multiome | 533 | |
| chr9:137,236,161–137,237,143 | 18.7 kb | Distal (>10kb) Multiome | 748 | |
| chr9:137,239,991–137,242,933 | 14.3 kb | Distal (>10kb) Multiome | 1117 | |
| chr9:137,243,659–137,245,307 | 11.0 kb | Distal (>10kb) Multiome | 471 | |
| chr9:137,252,677–137,252,835 | 2.5 kb | Proximal (<10kb) | 65 | |
| chr9:137,253,801–137,256,036 | 29 bp | At TSS Multiome | 843 | |
| chr9:137,280,593–137,281,861 | 25.9 kb | Distal (>10kb) Multiome | 223 | |
| chr9:137,294,859–137,296,916 | 40.2 kb | Distal (>10kb) Multiome | 501 | |
| chr9:137,300,591–137,303,141 | 47.1 kb | Distal (>10kb) Multiome | 790 | |
| chr9:137,316,104–137,316,916 | 61.0 kb | Distal (>10kb) Multiome | 395 | |
| chr9:137,406,843–137,407,556 | 151.8 kb | Distal (>10kb) Multiome | 427 | |
| chr9:137,422,664–137,424,024 | 168.0 kb | Distal (>10kb) Multiome | 896 | |
| chr9:137,441,270–137,441,970 | 186.2 kb | Distal (>10kb) Multiome | 568 | |
| chr9:137,453,153–137,455,543 | 198.7 kb | Distal (>10kb) Multiome | 727 | |
| chr9:137,458,468–137,459,887 | 204.0 kb | Distal (>10kb) Multiome | 613 | |
| chr9:137,461,555–137,462,160 | 206.6 kb | Distal (>10kb) Multiome | 284 | |
| chr9:137,551,136–137,552,512 | 296.4 kb | Distal (>10kb) Multiome | 756 |
Genomic view of the NELFB locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.