This gene encodes a nebulin like protein that is abundantly expressed in cardiac muscle. The encoded protein binds actin and interacts with thin filaments and Z-line associated proteins in striated muscle. This protein may be involved in cardiac myofibril assembly. A shorter isoform of this protein termed LIM nebulette is expressed in non-muscle cells and may function as a component of focal adhesion complexes. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
Transcription factors with Perturb-seq knockdown data for NEBL. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NEBL upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NEBL, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:21,173,016–21,174,917 | 118.8 kb | Distal (>10kb) Multiome | 549 | |
| chr10:21,281,583–21,282,796 | 10.8 kb | Distal (>10kb) Multiome | 230 | |
| chr10:21,285,276–21,285,425 | 7.6 kb | Proximal (<10kb) | 13 | |
| chr10:21,292,852–21,293,476 | at TSS | At TSS | 420 | |
| chr10:21,293,863–21,294,417 | 852 bp | At TSS | 148 | |
| chr10:21,313,840–21,314,525 | 21.3 kb | Distal (>10kb) Multiome | 145 | |
| chr10:21,417,277–21,417,788 | 124.4 kb | Distal (>10kb) Multiome | 150 | |
| chr10:21,495,254–21,497,513 | 203.0 kb | Distal (>10kb) Multiome HiCAR | 1097 | |
| chr10:21,508,081–21,511,137 | 217.6 kb | Distal (>10kb) Multiome | 373 | |
| chr10:21,515,560–21,517,745 | 223.1 kb | Distal (>10kb) Multiome | 690 | |
| chr10:21,524,213–21,526,810 | 233.1 kb | Distal (>10kb) Multiome | 1100 | |
| chr10:21,527,043–21,527,629 | 234.4 kb | Distal (>10kb) Multiome | 673 | |
| chr10:21,533,309–21,535,662 | 241.1 kb | Distal (>10kb) Multiome | 1089 |
Genomic view of the NEBL locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.