This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq, Mar 2010]
Transcription factors with Perturb-seq knockdown data for NDUFS8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NDUFS8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NDUFS8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:67,757,971–67,758,804 | 272.3 kb | Distal (>10kb) Multiome | 61 | |
| chr11:67,804,576–67,806,617 | 225.0 kb | Distal (>10kb) Multiome | 372 | |
| chr11:67,955,492–67,956,004 | 74.9 kb | Distal (>10kb) Multiome | 140 | |
| chr11:67,990,829–67,991,405 | 39.6 kb | Distal (>10kb) Multiome | 54 | |
| chr11:67,996,583–67,997,678 | 33.6 kb | Distal (>10kb) Multiome | 394 | |
| chr11:68,002,991–68,004,268 | 26.6 kb | Distal (>10kb) Multiome | 634 | |
| chr11:68,030,139–68,031,153 | 69 bp | At TSS Multiome | 708 | |
| chr11:68,038,378–68,039,498 | 8.3 kb | Proximal (<10kb) Multiome | 819 | |
| chr11:68,040,369–68,040,559 | 9.7 kb | Proximal (<10kb) | 412 | |
| chr11:68,120,465–68,122,314 | 90.9 kb | Distal (>10kb) Multiome | 921 | |
| chr11:68,127,759–68,129,017 | 97.8 kb | Distal (>10kb) Multiome | 604 | |
| chr11:68,212,619–68,214,642 | 182.3 kb | Distal (>10kb) Multiome | 967 | |
| chr11:68,271,213–68,272,543 | 241.4 kb | Distal (>10kb) Multiome | 943 | |
| chr11:68,312,404–68,312,863 | 282.0 kb | Distal (>10kb) Multiome | 279 |
Genomic view of the NDUFS8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.