NDUFS5

NADH:ubiquinone oxidoreductase subunit S5 | CI-15k

This gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants and pseudogenes have been identified on chromosomes 1, 4 and 17. [provided by RefSeq, May 2010]

Member of: DE-1 Developmental clusters: GC5

Biological processes 19 terms

NADH dehydrogenase (ubiquinone) activity (GO:0008137)aerobic respiration (GO:0009060)mitochondrial electron transport, NADH to ubiquinone (GO:0006120)mitochondrial inner membrane (GO:0005743)mitochondrial inner membrane (GO:0005743)mitochondrial inner membrane (GO:0005743)mitochondrial inner membrane (GO:0005743)mitochondrial intermembrane space (GO:0005758)mitochondrial respiratory chain complex I assembly (GO:0032981)mitochondrial respiratory chain complex I assembly (GO:0032981)mitochondrion (GO:0005739)mitochondrion (GO:0005739)protein binding (GO:0005515)proton motive force-driven mitochondrial ATP synthesis (GO:0042776)proton transmembrane transport (GO:1902600)respiratory chain complex I (GO:0045271)respiratory chain complex I (GO:0045271)respiratory chain complex I (GO:0045271)respiratory chain complex I (GO:0045271)

Expression (TPM)

TFs regulating NDUFS5 0 TFs

Transcription factors with Perturb-seq knockdown data for NDUFS5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NDUFS5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to NDUFS5

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NDUFS5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr1:38,803,885–38,804,528	222.1 kb	Distal (>10kb) Multiome	257
chr1:38,817,720–38,818,696	208.1 kb	Distal (>10kb) Multiome	375
chr1:38,859,087–38,860,584	166.5 kb	Distal (>10kb) Multiome	848
chr1:38,872,771–38,873,884	152.9 kb	Distal (>10kb) Multiome	778
chr1:38,990,541–38,992,051	35.2 kb	Distal (>10kb) Multiome	902
chr1:39,025,981–39,026,877	44 bp	At TSS Multiome	808
chr1:39,080,764–39,081,936	54.9 kb	Distal (>10kb) Multiome	352
chr1:39,104,780–39,106,278	79.4 kb	Distal (>10kb) Multiome	621
chr1:39,132,955–39,133,390	106.9 kb	Distal (>10kb) Multiome	384
chr1:39,276,096–39,276,654	250.1 kb	Distal (>10kb) Multiome	93

Genome Browser

Genomic view of the NDUFS5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.