This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which is the first enzyme in the electron transport chain of mitochondria. This protein localizes to the inner membrane of the mitochondrion as a single-pass membrane protein. Mutations in this gene contribute to mitochondrial complex 1 deficiency. Alternative splicing results in multiple transcript variants encoding the same protein. Humans have multiple pseudogenes of this gene. [provided by RefSeq, Mar 2012]
Transcription factors with Perturb-seq knockdown data for NDUFB3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NDUFB3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NDUFB3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:200,810,854–200,812,782 | 260.4 kb | Distal (>10kb) Multiome | 1048 | |
| chr2:200,863,766–200,865,261 | 207.3 kb | Distal (>10kb) Multiome | 1111 | |
| chr2:200,888,532–200,889,950 | 182.7 kb | Distal (>10kb) Multiome | 1108 | |
| chr2:200,962,915–200,964,242 | 108.4 kb | Distal (>10kb) Multiome | 1021 | |
| chr2:201,071,202–201,072,338 | 193 bp | At TSS Multiome | 993 | |
| chr2:201,090,426–201,091,071 | 18.8 kb | Distal (>10kb) Multiome | 68 | |
| chr2:201,116,279–201,117,190 | 44.7 kb | Distal (>10kb) Multiome | 907 | |
| chr2:201,118,264–201,119,153 | 46.7 kb | Distal (>10kb) Multiome | 863 | |
| chr2:201,125,205–201,126,756 | 54.4 kb | Distal (>10kb) Multiome | 696 | |
| chr2:201,157,926–201,158,657 | 86.4 kb | Distal (>10kb) Multiome | 540 | |
| chr2:201,182,711–201,183,380 | 111.0 kb | Distal (>10kb) Multiome | 624 |
Genomic view of the NDUFB3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.