The protein encoded by this gene belongs to the complex I 19 kDa subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
Transcription factors with Perturb-seq knockdown data for NDUFA8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NDUFA8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NDUFA8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:122,092,682–122,093,762 | 66.4 kb | Distal (>10kb) Multiome | 771 | |
| chr9:122,099,169–122,100,386 | 60.0 kb | Distal (>10kb) Multiome | 262 | |
| chr9:122,158,300–122,158,521 | 1.3 kb | Proximal (<10kb) | 144 | |
| chr9:122,159,157–122,160,157 | 29 bp | At TSS Multiome | 592 | |
| chr9:122,213,553–122,214,655 | 54.3 kb | Distal (>10kb) Multiome | 629 | |
| chr9:122,219,092–122,220,535 | 59.7 kb | Distal (>10kb) Multiome | 158 | |
| chr9:122,228,256–122,229,326 | 69.0 kb | Distal (>10kb) Multiome | 307 | |
| chr9:122,264,447–122,265,544 | 105.1 kb | Distal (>10kb) Multiome | 1042 | |
| chr9:122,405,953–122,406,784 | 246.6 kb | Distal (>10kb) Multiome | 118 |
Genomic view of the NDUFA8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.