This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that is required for cell cycle progression and survival in primary astrocytes and may be involved in the regulation of mitogenic signalling in vascular smooth muscles cells. Alternative splicing results in multiple transcripts encoding different isoforms.[provided by RefSeq, Jun 2011]
Transcription factors with Perturb-seq knockdown data for NDRG4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NDRG4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NDRG4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr16:58,197,509–58,198,705 | 265.2 kb | Distal (>10kb) Multiome | 490 | |
| chr16:58,236,247–58,236,852 | 226.8 kb | Distal (>10kb) Multiome | 355 | |
| chr16:58,249,584–58,250,557 | 213.4 kb | Distal (>10kb) Multiome | 641 | |
| chr16:58,392,269–58,393,124 | 70.7 kb | Distal (>10kb) Multiome | 993 | |
| chr16:58,463,143–58,465,363 | 311 bp | At TSS Multiome | 307 | |
| chr16:58,471,333–58,471,935 | 8.0 kb | Proximal (<10kb) | 102 | |
| chr16:58,487,547–58,488,283 | 24.5 kb | Distal (>10kb) Multiome | 523 | |
| chr16:58,499,699–58,500,457 | 36.6 kb | Distal (>10kb) Multiome | 283 | |
| chr16:58,500,548–58,502,121 | 38.1 kb | Distal (>10kb) Multiome | 481 | |
| chr16:58,515,027–58,516,681 | 52.2 kb | Distal (>10kb) Multiome | 1010 | |
| chr16:58,629,310–58,630,709 | 166.8 kb | Distal (>10kb) Multiome | 1050 | |
| chr16:58,684,159–58,685,101 | 221.4 kb | Distal (>10kb) Multiome | 695 | |
| chr16:58,733,415–58,734,721 | 270.9 kb | Distal (>10kb) Multiome | 1031 | |
| chr16:58,756,000–58,756,694 | 293.1 kb | Distal (>10kb) Multiome | 352 |
Genomic view of the NDRG4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.