This gene was identified by its close proximity on chromosome 17 to tumor suppressor gene BRCA1. Experimental evidence indicates that the two genes share a bi-directional promoter. Transcription for either gene is controlled individually by distinct transcriptional repressor factors. A short (112 amino acid) open reading frame is observed which includes a region derived from a LINE1 element. A strong Kozak signal is not observed for the putative ORF and the stop codon is more than 55 nucleotides upstream of the last splice site for the transcript, suggesting that the transcript is subject to nonsense-mediated decay. Therefore, this gene does not appear to encode a protein. Glucose starvation induces the expression of this gene and the long non-coding RNA transcribed by it functions with AMP-activated protein kinase in mediating the energy stress response. [provided by RefSeq, Aug 2016]
Transcription factors with Perturb-seq knockdown data for NBR2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NBR2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NBR2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:42,832,889–42,833,841 | 292.3 kb | Distal (>10kb) Multiome | 980 | |
| chr17:42,964,237–42,964,843 | 161.1 kb | Distal (>10kb) Multiome | 655 | |
| chr17:42,979,966–42,981,044 | 145.2 kb | Distal (>10kb) Multiome | 843 | |
| chr17:42,997,598–42,998,917 | 127.3 kb | Distal (>10kb) Multiome | 1112 | |
| chr17:43,021,026–43,022,568 | 103.3 kb | Distal (>10kb) Multiome | 973 | |
| chr17:43,024,255–43,025,752 | 100.6 kb | Distal (>10kb) Multiome | 811 | |
| chr17:43,124,981–43,125,702 | 156 bp | At TSS Multiome | 837 | |
| chr17:43,169,744–43,171,576 | 45.5 kb | Distal (>10kb) Multiome | 1018 | |
| chr17:43,211,494–43,212,392 | 86.3 kb | Distal (>10kb) Multiome | 586 | |
| chr17:43,359,784–43,361,254 | 235.4 kb | Distal (>10kb) Multiome | 981 | |
| chr17:43,367,132–43,369,458 | 243.5 kb | Distal (>10kb) Multiome | 1146 | |
| chr17:43,388,101–43,389,658 | 263.7 kb | Distal (>10kb) Multiome | 770 | |
| chr17:43,396,555–43,396,987 | 271.1 kb | Distal (>10kb) Multiome | 27 | |
| chr17:43,398,359–43,400,469 | 273.3 kb | Distal (>10kb) Multiome | 827 | |
| chr17:43,403,154–43,404,714 | 278.3 kb | Distal (>10kb) Multiome | 252 | |
| chr17:43,406,748–43,407,604 | 281.5 kb | Distal (>10kb) Multiome | 102 |
Genomic view of the NBR2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.