This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013]
Transcription factors with Perturb-seq knockdown data for NBL1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NBL1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NBL1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:19,311,493–19,312,976 | 284.8 kb | Distal (>10kb) Multiome | 868 | |
| chr1:19,338,257–19,338,718 | 258.4 kb | Distal (>10kb) Multiome | 426 | |
| chr1:19,484,474–19,486,386 | 111.3 kb | Distal (>10kb) Multiome | 809 | |
| chr1:19,596,392–19,597,600 | 5 bp | At TSS Multiome | 888 | |
| chr1:19,640,074–19,641,053 | 43.6 kb | Distal (>10kb) Multiome | 418 | |
| chr1:19,643,702–19,645,210 | 47.2 kb | Distal (>10kb) Multiome | 427 | |
| chr1:19,653,578–19,654,269 | 57.0 kb | Distal (>10kb) Multiome | 321 | |
| chr1:19,659,723–19,660,209 | 63.0 kb | Distal (>10kb) Multiome | 316 | |
| chr1:19,704,357–19,704,828 | 107.7 kb | Distal (>10kb) Multiome | 244 | |
| chr1:19,882,144–19,883,038 | 285.4 kb | Distal (>10kb) Multiome | 815 |
Genomic view of the NBL1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.