This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for NANS. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NANS upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NANS, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:97,803,235–97,804,055 | 253.1 kb | Distal (>10kb) Multiome | 679 | |
| chr9:97,853,103–97,855,002 | 202.7 kb | Distal (>10kb) Multiome | 323 | |
| chr9:97,922,022–97,923,085 | 134.3 kb | Distal (>10kb) Multiome | 1110 | |
| chr9:97,982,380–97,985,451 | 72.0 kb | Distal (>10kb) Multiome | 994 | |
| chr9:98,056,283–98,057,271 | 73 bp | At TSS Multiome | 868 | |
| chr9:98,087,291–98,088,183 | 31.0 kb | Distal (>10kb) Multiome | 281 | |
| chr9:98,118,693–98,119,508 | 62.4 kb | Distal (>10kb) Multiome | 752 | |
| chr9:98,191,695–98,193,531 | 136.3 kb | Distal (>10kb) Multiome | 795 | |
| chr9:98,218,786–98,219,246 | 162.3 kb | Distal (>10kb) Multiome | 178 | |
| chr9:98,255,174–98,256,530 | 199.1 kb | Distal (>10kb) Multiome | 905 | |
| chr9:98,265,141–98,265,693 | 208.8 kb | Distal (>10kb) Multiome | 26 | |
| chr9:98,280,310–98,281,125 | 224.1 kb | Distal (>10kb) Multiome | 235 | |
| chr9:98,315,414–98,316,053 | 259.0 kb | Distal (>10kb) Multiome | 373 |
Genomic view of the NANS locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.