Nicotinamide adenine dinucleotide (NAD) is a coenzyme in metabolic redox reactions, a precursor for several cell signaling molecules, and a substrate for protein posttranslational modifications. NAD synthetase (EC 6.3.5.1) catalyzes the final step in the biosynthesis of NAD from nicotinic acid adenine dinucleotide (NaAD).[supplied by OMIM, Apr 2004]
Transcription factors with Perturb-seq knockdown data for NADSYN1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NADSYN1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NADSYN1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:71,246,399–71,246,829 | 206.7 kb | Distal (>10kb) Multiome | 102 | |
| chr11:71,251,249–71,252,925 | 201.4 kb | Distal (>10kb) Multiome | 518 | |
| chr11:71,447,762–71,449,172 | 4.8 kb | Proximal (<10kb) Multiome | 802 | |
| chr11:71,451,905–71,452,114 | 1.1 kb | Proximal (<10kb) | 51 | |
| chr11:71,452,706–71,453,785 | 92 bp | At TSS Multiome | 636 | |
| chr11:71,487,897–71,488,597 | 35.1 kb | Distal (>10kb) Multiome | 181 | |
| chr11:71,607,766–71,608,303 | 154.7 kb | Distal (>10kb) Multiome | 13 | |
| chr11:71,750,971–71,751,686 | 298.0 kb | Distal (>10kb) Multiome | 86 |
Genomic view of the NADSYN1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.