This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes. [provided by RefSeq, Feb 2016]
Transcription factors with Perturb-seq knockdown data for NACA. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = NACA upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of NACA, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:56,448,848–56,449,756 | 276.1 kb | Distal (>10kb) Multiome | 920 | |
| chr12:56,468,239–56,468,840 | 256.8 kb | Distal (>10kb) Multiome | 907 | |
| chr12:56,487,633–56,488,590 | 237.0 kb | Distal (>10kb) Multiome | 784 | |
| chr12:56,629,725–56,630,674 | 95.0 kb | Distal (>10kb) Multiome | 759 | |
| chr12:56,633,838–56,637,505 | 91.0 kb | Distal (>10kb) Multiome | 1037 | |
| chr12:56,645,530–56,646,466 | 79.2 kb | Distal (>10kb) Multiome | 902 | |
| chr12:56,687,276–56,689,898 | 35.7 kb | Distal (>10kb) Multiome | 1162 | |
| chr12:56,724,695–56,725,713 | 1 bp | At TSS Multiome | 972 | |
| chr12:56,751,814–56,752,566 | 26.9 kb | Distal (>10kb) Multiome | 765 | |
| chr12:57,006,056–57,006,942 | 281.2 kb | Distal (>10kb) Multiome | 422 |
Genomic view of the NACA locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.