Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for MRPS28. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = MRPS28 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of MRPS28, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:79,765,078–79,765,878 | 264.6 kb | Distal (>10kb) Multiome HiCAR | 627 | |
| chr8:79,767,221–79,769,084 | 261.9 kb | Distal (>10kb) Multiome HiCAR | 776 | |
| chr8:79,783,349–79,784,558 | 246.0 kb | Distal (>10kb) Multiome | 708 | |
| chr8:79,890,536–79,892,143 | 139.0 kb | Distal (>10kb) Multiome | 606 | |
| chr8:80,023,687–80,024,081 | 5.9 kb | Proximal (<10kb) | 167 | |
| chr8:80,029,587–80,030,640 | 164 bp | At TSS Multiome | 989 | |
| chr8:80,170,981–80,171,950 | 141.5 kb | Distal (>10kb) Multiome | 771 | |
| chr8:80,211,280–80,212,758 | 181.9 kb | Distal (>10kb) Multiome | 216 | |
| chr8:80,231,585–80,232,350 | 202.1 kb | Distal (>10kb) Multiome | 124 | |
| chr8:80,240,457–80,241,089 | 210.8 kb | Distal (>10kb) Multiome | 564 | |
| chr8:80,279,352–80,280,425 | 249.8 kb | Distal (>10kb) Multiome | 186 |
Genomic view of the MRPS28 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.